Wu Yali, Chen Wenjuan, Deng Jingjing, Cao Xinghui, Yang Zimo, Chen Jiangbin, Tan Qi, Zhou E, Li Minglei, Liu Jiatong, Guo Mengfei, Jin Yang
Department of Respiratory and Critical Care Medicine, Hubei Province Clinical Research Center for Major Respiratory Diseases, NHC Key Laboratory of Pulmonary Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Hubei Province Key Laboratory of Biological Targeted Therapy, MOE Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Nat Nanotechnol. 2025 May 19. doi: 10.1038/s41565-025-01922-3.
Tumour-derived microparticles (TMPs), extracellular vesicles traditionally obtained upon ultraviolet (UV) radiation of tumour cells, hold promise in tumour immunotherapies and vaccines and have demonstrated potential as drug delivery systems for tumour treatment. However, concerns remain regarding the limited efficacy and safety of UV-derived TMPs. Here we introduce a microwave (MW)-assisted method for preparing TMPs, termed MW-TMPs. Brief exposure of tumour cells to short-wavelength MW radiation promotes the release of TMPs showing superior in vivo antitumour activity and safety compared with UV-TMPs. MW-TMPs induce immunogenic cell death and reprogramme suppressive tumour immune microenvironments in different lung tumour models, enabling dual targeting of tumour cells by natural killer and T cells. We show that they can efficiently deliver methotrexate to tumours, synergistically boosting the efficacy of PD-L1 blockade. This MW-TMP development strategy is simpler, more efficient and safer than traditional UV-TMP methods.
肿瘤衍生的微粒（TMPs）是传统上通过对肿瘤细胞进行紫外线（UV）辐射而获得的细胞外囊泡，在肿瘤免疫疗法和疫苗方面具有前景，并已证明有潜力作为肿瘤治疗的药物递送系统。然而，对于紫外线衍生的TMPs的有限疗效和安全性仍存在担忧。在此，我们介绍一种用于制备TMPs的微波（MW）辅助方法，称为MW-TMPs。将肿瘤细胞短暂暴露于短波长MW辐射下可促进TMPs的释放，与UV-TMPs相比，MW-TMPs在体内显示出卓越的抗肿瘤活性和安全性。在不同的肺肿瘤模型中，MW-TMPs诱导免疫原性细胞死亡并重新编程抑制性肿瘤免疫微环境，使自然杀伤细胞和T细胞能够双重靶向肿瘤细胞。我们表明，它们可以有效地将甲氨蝶呤递送至肿瘤，协同增强PD-L1阻断的疗效。这种MW-TMP的开发策略比传统的UV-TMP方法更简单、更高效且更安全。
