Watts L K, Duffy P, Field R B, Stafford E G, O'Brien M F
Ann Thorac Surg. 1976 Mar;21(3):230-6. doi: 10.1016/s0003-4975(10)64297-x.
A method for determining the viability of homograft valves has been developed based on sequential measurements of glucose and pH levels of the culture medium in which cardiac valves have been maintained for short periods at 37 degrees C. Viable valves, as determined by tissue culture, showed a characteristic pattern of glucose utilization and pH reduction that was absent in nonviable valves. Upon explantation of valve leaflet fragments into tissue culture, only fragments from valves that metabolized glucose produced viable fibroblast cultures. The method reported here is rapid, requires no specialized equipment, is nondestructive, and can directly determine the viability of the valve homograft within 24 to 48 hours.
基于对培养基中葡萄糖和pH值的连续测量，已开发出一种确定同种异体移植瓣膜活力的方法。在37摄氏度下，心脏瓣膜在该培养基中短期保存。通过组织培养确定，有活力的瓣膜呈现出一种葡萄糖利用和pH值降低的特征模式，而无活力的瓣膜则没有这种模式。将瓣膜小叶碎片植入组织培养后，只有来自代谢葡萄糖的瓣膜的碎片才能产生有活力的成纤维细胞培养物。此处报道的方法快速，不需要专门设备，是非破坏性的，并且可以在24至48小时内直接确定瓣膜同种异体移植的活力。
