Yi Chenyang, Xu Qiaoxia, Han Yu, Deng Mingyong, Li Guohong, Li Chao, Sun Xiaomei, Zhong Ming, Jin Meilin, Kang Chao
Wuhan Keqian Biology Co., Ltd, Wuhan, Hubei, 430070, China.
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Poult Sci. 2025 Aug;104(8):105248. doi: 10.1016/j.psj.2025.105248. Epub 2025 May 10.
Mycoplasma synoviae (MS) infection predominantly manifests as lower respiratory symptoms but also leads to reduced egg production and increased culling rates in laying hens, causing significant economic losses throughout the production cycle. Vaccination remains the most cost-effective strategy to protect layers from MS infection. Previous studies from our laboratory identified and screened immunogenic proteins of MS, culminating in the development of a subunit vaccine that provided robust protection with a two-dose immunization. Building on these findings, this study evaluated various formulations, antigen combinations, and adjuvants of MS inactivated vaccines, tailored to meet field immunization protocols and disease prevention needs. The results indicated that an inactivated vaccine combining whole-cell and subunit components of MS offered effective protection in layers during the pullet-rearing phase with a single immunization. Interestingly, vaccine-induced antibody levels did not strongly correlate with protective efficacy, underscoring the importance of host cellular immunity in combating MS infection. In conclusion, this study addresses field-relevant challenges and presents innovative strategies for MS control in laying hens, offering practical insights for improving disease control and flock management.
鸡滑液支原体（MS）感染主要表现为下呼吸道症状，但也会导致蛋鸡产蛋量下降和淘汰率增加，在整个生产周期中造成重大经济损失。接种疫苗仍然是保护蛋鸡免受MS感染的最具成本效益的策略。我们实验室之前的研究鉴定并筛选了MS的免疫原性蛋白，最终开发出一种亚单位疫苗，通过两剂免疫提供了强大的保护作用。基于这些发现，本研究评估了MS灭活疫苗的各种配方、抗原组合和佐剂，以满足现场免疫方案和疾病预防需求。结果表明，一种将MS全细胞和亚单位成分结合的灭活疫苗在育雏期对蛋鸡进行单次免疫即可提供有效的保护。有趣的是，疫苗诱导的抗体水平与保护效力并没有很强的相关性，这突出了宿主细胞免疫在对抗MS感染中的重要性。总之，本研究解决了与现场相关的挑战，并提出了蛋鸡MS防控的创新策略，为改善疾病控制和鸡群管理提供了实用见解。
