Abro Aamir Ali, Sun Cong, Abbas Mubashir, Liu Qiankun, Jie Zheng, Xu Yanchao, Hou Yuqing, Zhou Zhongli, Iqbal Rashid, Liu Fang, Cai Xiaoyan
Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences (ICR, CAAS), Anyang, 455000, Henan, People's Republic of China.
Department of Agronomy, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur Pakistan, Bahawalpur, 63100, Pakistan.
Funct Integr Genomics. 2025 May 20;25(1):104. doi: 10.1007/s10142-025-01618-w.
Bcl-2-associated athanogene (BAG) gene family is important in stress tolerance and death regulation in plants. Cotton (Gossypium hirsutum) is an important cash crop with strong functional significance, while the BAG gene family in cotton has been little studied. Remains largely unexplored. Of these, the genome-wide identification and characterization of BAG genes were performed in Gossypium hirsutum, Gossypium barbadense, Gossypium raimondii, and Gossypium arboreum in this study. In G. hirsutum, G. barbadense, G. raimondii, and G. arboreum, there were 30, 32, 12, and 11 BAG genes found, respectively. Phylogenetic classification groupgrouped these genes into five classes (A-E), depending on their evolutionary relatedness with the BAG genes from other plant species. Investigation of the gene structures and expression patterns of BAG proteins indicated conserved domain architectures, gene motifs, and subcellular localizations among Gossypium species. Within tetraploid species, whole-genome and segmental duplications were determined to be the main contributors to BAG gene expansion, while diploid progenitors had few gene duplication events. Comparative sequence approaches and analyses of conserved motifs revealed jagged evolutionary conservation of the BAG domain indicating their possible functional roles in stress response and programmed cell death. Detailed expression profiling under abiotic stress conditions (drought, salt, and cold) showed that several BAG genes significantly differentially expressed which indicating their participation in adaptation mechanisms to stress conditions. In addition, the identification of essential cis-regulatory factors in the promoter regions suggested potential regulation by environmental changes. BAG gene family Structure, evolutionary relationship and expression pattern in cotton in response to different stresses study with predictable implications. Deciphering how the BAG gene functions at a molecular and evolutionary scale will help guide future research into genetic engineering approaches aimed at enhancing cotton tolerance to environmental stressors.
Bcl-2相关抗凋亡基因（BAG）基因家族在植物的胁迫耐受性和死亡调控中具有重要作用。棉花（陆地棉）是一种具有重要功能意义的重要经济作物，而棉花中的BAG基因家族研究较少。在很大程度上仍未被探索。在本研究中，对陆地棉、海岛棉、雷蒙德氏棉和亚洲棉中的BAG基因进行了全基因组鉴定和特征分析。在陆地棉、海岛棉、雷蒙德氏棉和亚洲棉中，分别发现了30个、32个、12个和11个BAG基因。系统发育分类根据这些基因与其他植物物种的BAG基因的进化相关性将它们分为五类（A - E）。对BAG蛋白的基因结构和表达模式的研究表明，棉属物种之间存在保守的结构域结构、基因基序和亚细胞定位。在四倍体物种中，全基因组和片段重复被确定为BAG基因扩增的主要因素，而二倍体祖先的基因重复事件较少。比较序列方法和保守基序分析揭示了BAG结构域的锯齿状进化保守性，表明它们在应激反应和程序性细胞死亡中可能发挥的功能作用。在非生物胁迫条件（干旱、盐和冷）下的详细表达谱分析表明，几个BAG基因显著差异表达，这表明它们参与了对胁迫条件的适应机制。此外，对启动子区域中必需顺式调控因子的鉴定表明可能受环境变化的调控。棉花中BAG基因家族的结构、进化关系和表达模式对不同胁迫的响应研究具有可预测的意义。在分子和进化尺度上解读BAG基因的功能将有助于指导未来旨在提高棉花对环境胁迫耐受性的基因工程方法的研究。
