Yamazaki M, Kisugi J, Kimura K, Kamiya H, Mizuno D
FEBS Lett. 1985 Jun 17;185(2):295-8. doi: 10.1016/0014-5793(85)80926-1.
An antineoplastic factor inducing tumor lysis was purified to apparent homogeneity from the supernatant of a homogenate of eggs of the sea hare Aplysia kurodai. On gel filtration, the purified substance gave a single band of material of 250 kDa containing 3 different subunits and this band coincided with cytolytic activity. The purified factor was half-maximally active at 10-60 ng protein/ml and lysed all tumor cells tested, but not normal cells.
从黑指纹海兔（Aplysia kurodai）卵匀浆的上清液中纯化出一种诱导肿瘤溶解的抗肿瘤因子，达到了表观均一性。在凝胶过滤中，纯化后的物质呈现出一条250 kDa的单一蛋白带，包含3种不同的亚基，且这条带与细胞溶解活性一致。纯化后的因子在10 - 60 ng蛋白质/毫升时活性达到半数最大值，能裂解所有测试的肿瘤细胞，但对正常细胞无作用。
