使用原子力显微镜测量类器官杨氏模量的实验方案。
Protocol for measuring the Young's modulus of organoids using atomic force microscopy.
作者信息
Zhang Tianzhen, Ma Lina, Ling Shen, Chen Yupeng, Zhang Zhongtao, Tian Dan, Yang Yingchi
机构信息
Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; State Key Lab of Digestive Health, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; National Clinical Research Center for Digestive Diseases, Beijing 100050, China.
Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; State Key Lab of Digestive Health, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; National Clinical Research Center for Digestive Diseases, Beijing 100050, China; Immunology Research Center for Oral and Systemic Health, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
出版信息
STAR Protoc. 2025 Jun 20;6(2):103825. doi: 10.1016/j.xpro.2025.103825. Epub 2025 May 20.
Atomic force microscopy (AFM) is extensively applied to measure cell and tissue mechanics but lacks a standardized organoid stiffness assessment. Here, we present a protocol for quantifying the Young's modulus of organoids via AFM, combining force-curve analysis with an optimized probe. We describe steps for preparing organoids, OCT embedding, slicing, AFM detection, and force-curve analysis. By mechanically addressing gaps in organoids, this protocol improves reproducibility and expands the capabilities of biomechanical research.
原子力显微镜（AFM）被广泛应用于测量细胞和组织力学，但缺乏标准化的类器官硬度评估方法。在此，我们提出了一种通过AFM定量测定类器官杨氏模量的方案，将力曲线分析与优化的探针相结合。我们描述了类器官制备、OCT包埋、切片、AFM检测和力曲线分析的步骤。通过机械解决类器官中的差距，该方案提高了可重复性并扩展了生物力学研究的能力。
Zotero 插件