使用原子力显微镜测量小鼠心肌细胞膜弹性的实验方案。
Protocol for measuring membrane elasticity of mouse cardiomyocytes using atomic force microscopy.
作者信息
Morales Maldonado Andrés David, Diloretto Daphne Agostina, Timofeyev Valeriy, Karsai Arpad, Ogorodnik Evgeny, Huang Yuqi, Zong Ning, Liu Gang-Yu, Chiamvimonvat Nipavan, Zhang Xiao-Dong
机构信息
Department of Chemistry, University of California, Davis, Davis, CA 95616, USA.
Department of Internal Medicine, University of California, Davis, Davis, CA 95616, USA.
出版信息
STAR Protoc. 2025 Apr 11;6(2):103746. doi: 10.1016/j.xpro.2025.103746.
Atomic force microscopy (AFM) is a powerful technique that enables the determination of cellular mechanics at the single-cell level. Here, we present a protocol for using AFM to measure membrane mechanical properties of mouse ventricular cardiomyocytes. The key steps include isolation of mouse cardiomyocytes, cantilever preparation, modification and calibration, and measurement of force-deformation profiles, from which membrane Young's moduli are quantified. The outcomes advance our knowledge of cardiomyocyte's unique mechanical and dynamic properties.
原子力显微镜（AFM）是一种强大的技术，能够在单细胞水平上测定细胞力学。在此，我们展示了一种使用AFM测量小鼠心室心肌细胞膜力学特性的方案。关键步骤包括小鼠心肌细胞的分离、悬臂梁制备、修饰和校准，以及力-变形曲线的测量，从中可以量化膜的杨氏模量。这些结果推进了我们对心肌细胞独特力学和动态特性的认识。
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