脑特异性蛋白14-3-2的制备与性质

Preparation and properties of the brain specific protein 14-3-2.

作者信息

Grasso A, Roda G, Hogue-Angeletti R A, Moore B W, Perez V J

出版信息

Brain Res. 1977 Apr 1;124(3):497-507. doi: 10.1016/0006-8993(77)90949-0.

Abstract

A brain specific protein, 14-3-2, has been isolated from bovine brain by the use of ammonium sulfate precipitation, gel filtration on Sephadex G-150, and ion exchange chromatography on DEAE-cellulose and DEAE-Sephadex. It is an acidic protein, in agreement with the high content of glutamic and aspartic acid, that is composed of a single polypeptide chain of mol. wt. 50,000. Immunochemical tests using antiserum to purified 14-3-2 showed that the protein is present in at least 100-fold greater amounts in brain than in any other rat tissue. Furthermore, 14-3-2 was found in brains of a number of vertebrate species, although the antigen is apparently not entirely identical in all species tested. The protein 14-3-2 can be considered to be a species non-specific protein which is neuronal in origin.

摘要

一种脑特异性蛋白质14 - 3 - 2已通过硫酸铵沉淀、Sephadex G - 150凝胶过滤以及DEAE - 纤维素和DEAE - 葡聚糖离子交换色谱法从牛脑中分离出来。它是一种酸性蛋白质，这与谷氨酸和天冬氨酸的高含量相符，由一条分子量为50,000的单多肽链组成。使用针对纯化的14 - 3 - 2的抗血清进行的免疫化学测试表明，该蛋白质在脑中的含量至少比任何其他大鼠组织高100倍。此外，在许多脊椎动物物种的脑中都发现了14 - 3 - 2，尽管在所有测试物种中该抗原显然并不完全相同。蛋白质14 - 3 - 2可被认为是一种起源于神经元的物种非特异性蛋白质。