De Vitry F, Picart R, Jacque C, Legault L, Dupouey P, Tixier-Vidal A
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4165-9. doi: 10.1073/pnas.77.7.4165.
The cellular localization of a neuronal and a glial cell specific protein (14-3-2 and S-100, respectively) has been explored in mouse hypothalamus in order to trace cell lineages. This study was performed on fixed slices, at the light microscope level, by using either the indirect peroxidase-labeled immunoglobulin technique or immunofluorescence. In the adult, only S-100 immunoreactivity was found in the ependymal layer. In contrast, the magnocellular neurons of the preoptic area displayed strong 14-3-2 immunoreactivity. At neonatal stages (fetal day 17-postnatal day 3), both 14-3-2 and S-100 immunoreactivities developed simultaneously in the same cells lining the ventral part of the third ventricle. Transient detachment of some of these ventricular cells could be visualized before migration in the hypothalamus where they remained as bipotential cells up to postnatal day 10. Later in the development, they differentiated into separate cells, one type containing 14-3-2 and the other S-100, like neurons and glial cells. These results argue for a developmental stage during which cells lining the ventricle are bipotential and may thus be candidates for the role of stem cells for both neuronal and glial lineages.
为了追踪细胞谱系，研究了小鼠下丘脑神经元和神经胶质细胞特异性蛋白（分别为14-3-2和S-100）的细胞定位。本研究在固定切片上进行，在光学显微镜水平下，使用间接过氧化物酶标记免疫球蛋白技术或免疫荧光技术。在成年小鼠中，仅在室管膜层发现S-100免疫反应性。相反，视前区的大细胞神经元显示出强烈的14-3-2免疫反应性。在新生阶段（胚胎第17天-出生后第3天），14-3-2和S-100免疫反应性在第三脑室腹侧的同一细胞中同时出现。在这些脑室细胞迁移到下丘脑之前，可以观察到其中一些细胞的短暂脱离，它们在下丘脑一直保持双能细胞状态直至出生后第10天。在发育后期，它们分化为不同的细胞，一种含有14-3-2，另一种含有S-100，类似于神经元和神经胶质细胞。这些结果表明，在发育阶段，脑室衬里细胞具有双能性，因此可能是神经元和神经胶质谱系干细胞角色的候选者。
