Dongye Chenxin, Chen Xiangrong, Zhao Yanfang, Li Huijuan, Abdallah Mohamed F, Li Tianliang, Chen Xiangfeng
Shandong International Joint Laboratory of Environmental and Biological Science, Qilu University of Technology (Shandong Academy of Science), Jinan, Shandong 250014, China.
Department of Human Biology and Toxicology, Faculty of Medicine, Pharmacy and Biomedical Sciences, University of Mons, Mons 7000, Belgium.
Environ Health (Wash). 2025 Feb 6;3(5):515-525. doi: 10.1021/envhealth.4c00250. eCollection 2025 May 16.
Deoxynivalenol (DON) and octyl gallate (OG) are prevalent compounds in the environment and food. DON is frequently detected in cereals such as corn and wheat, while OG is commonly employed as a food additive. As a result, human exposure to these substances is inevitable. Given this, the objective of this experiment was to investigate the impact of co-exposure to DON (10 μg/kg) and OG (10 μg/kg) on intestinal inflammation. The RAW264.7 macrophage cell line was utilized to analyze cytokine levels as well as proteomic and metabolomic changes. In the quantitative real-time PCR experiments, the DON group showed significant difference compared to the control group (* < 0.05) and the DON-OG group (# < 0.05) regarding cytokine levels such as IL-10, TNF-α, Il6, Il1b, Ccl2, Il12α, Nos2, Cxcl1, and Cxcl2. In the animal experiment, C57BL/6 mice were utilized to monitor body weight, the presence of bloody stools, and diarrhea. Additionally, the colonic tissues of the mice underwent pathological analysis. The results indicated that cells treated with both DON and OG displayed lower levels of inflammation compared to those treated with DON alone. Furthermore, proteomic and metabolomic analyses revealed that the regulation of the Lancl2 protein and the mTOR signaling pathway contributed to the milder inflammatory response observed in the DON-OG group. These findings were further corroborated by the pathological analysis of the colonic tissues from the mice. In the combined exposure of DON and OG, OG partially mitigated the intestinal inflammation induced by DON.
脱氧雪腐镰刀菌烯醇（DON）和没食子酸辛酯（OG）是环境和食物中普遍存在的化合物。DON经常在玉米和小麦等谷物中被检测到，而OG通常被用作食品添加剂。因此，人类不可避免地会接触到这些物质。鉴于此，本实验的目的是研究同时暴露于DON（10μg/kg）和OG（10μg/kg）对肠道炎症的影响。利用RAW264.7巨噬细胞系分析细胞因子水平以及蛋白质组和代谢组的变化。在定量实时PCR实验中，DON组在IL-10、TNF-α、Il6、Il1b、Ccl2、Il12α、Nos2、Cxcl1和Cxcl2等细胞因子水平方面与对照组（*<0.05）和DON-OG组（#<0.05）相比存在显著差异。在动物实验中，使用C57BL/6小鼠监测体重、血便和腹泻情况。此外，对小鼠的结肠组织进行了病理分析。结果表明，与单独用DON处理的细胞相比，同时用DON和OG处理的细胞炎症水平较低。此外，蛋白质组和代谢组分析显示，Lancl2蛋白和mTOR信号通路的调节导致DON-OG组观察到的炎症反应较轻。小鼠结肠组织的病理分析进一步证实了这些发现。在DON和OG的联合暴露中，OG部分减轻了DON诱导的肠道炎症。
