Triglia D, Braa S S, Yonan C, Naughton G K
Marrow-Tech, Inc., La Jolla, California 92037.
In Vitro Cell Dev Biol. 1991 Mar;27A(3 Pt 1):239-44. doi: 10.1007/BF02630923.
A new three-dimensional human skin model consisting of several layers of actively dividing and metabolically active human neonatal foreskin-derived fibroblasts and epidermal keratinocytes grown on nylon mesh has been used to assess the in vitro toxicity of test agents from various classes. Utilizing a slight modification of the published neutral red viability assay for endpoint determination, we have assayed and obtained dose-dependent toxicity curves for test agents from the following classes: detergents (n = 15), alcohols (n = 5), metal chlorides (n = 10), perfumes and colognes (n = 5), shampoos (n = 4), conditioners (n = 3), moisturizers (n = 3), pesticides (n = 3), and antimicrobial preservatives (n = 4). Limited comparisons to in vivo ocular irritancy data with alcohols and detergents are encouraging. We have demonstrated the utility of this metabolically active dermal substrate containing naturally secreted collagen and other extracellular matrix proteins along with the neutral red viability assay for assessing the toxicity of a number of test agents from a variety of different classes with broad industrial applications.
一种新的三维人体皮肤模型，由生长在尼龙网上的几层具有活跃分裂和代谢活性的人新生儿包皮来源的成纤维细胞和表皮角质形成细胞组成，已被用于评估各类受试物的体外毒性。利用对已发表的中性红活力测定法进行的轻微修改来确定终点，我们已测定并获得了以下各类受试物的剂量依赖性毒性曲线：洗涤剂（n = 15）、醇类（n = 5）、金属氯化物（n = 10）、香水和古龙水（n = 5）、洗发水（n = 4）、护发素（n = 3）、保湿剂（n = 3）、农药（n = 3）和抗菌防腐剂（n = 4）。与醇类和洗涤剂的体内眼刺激性数据进行的有限比较令人鼓舞。我们已经证明了这种含有天然分泌的胶原蛋白和其他细胞外基质蛋白的代谢活跃的真皮基质以及中性红活力测定法在评估来自具有广泛工业应用的各种不同类别的多种受试物毒性方面的实用性。
