Mohankumar Amrutha Vellore, Priya Vernita, Anbalagan Sanjana, Radhakrishnan Vidya, Kandasamy Jayakrishna, Mekata Tohru, Raja Sudhakaran
Aquaculture Biotechnology Laboratory, School of Biosciences and Technology (SBST), Vellore Institute of Technology, Vellore, Tamil Nadu 632014 India.
VIT School of Agricultural Innovations and Advanced Learning (VAIAL), Vellore, Tamil Nadu 632014 India.
3 Biotech. 2025 Jun;15(6):178. doi: 10.1007/s13205-025-04337-y. Epub 2025 May 19.
Shrimp culture industries faced huge economic losses due to white spot disease (WSD) caused by the white spot syndrome virus (WSSV). Microalgae are unicellular and can be found in both freshwater and marine habitats. In recent days, microalgae such as species have been explored for treating WSSV using various protocols including transgenic algae, recombinant protein, and dsRNA. The development of dsRNA-expressing lines in has improved the efficiency of the new vectors p2xTRBL and pSS116, enabling the selection of transgenic cells without needing markers. Producing VP28 dsRNA to fight the white spot syndrome virus (WSSV) in shrimp has proven particularly advantageous. Due to the development of recombinant plasmids that express VP28 genes in microalgae, oral vaccinations against WSSV have proven highly effective and demonstrated great survival rates in shrimp. Moreover, salina transgenic strains have been modified to generate VP28 genes to shield crayfish from WSSV. Because of their antiviral qualities against WSSV, microalgae such as sp. and sp. have also been used and may be useful in managing other aquatic viral infections. Through oral vaccination using sustainable and environmentally friendly practices, these microalgae serve as a disease-control strategy in aquaculture, improving the financial standing and income of aquaculture farmers. All microalgae have antiviral properties and act against the white spot syndrome virus (WSSV).
对虾养殖业因感染由白斑综合征病毒（WSSV）引起的白斑病而遭受了巨大的经济损失。微藻是单细胞生物，在淡水和海洋环境中均有发现。近年来，人们探索了诸如某些物种的微藻，采用包括转基因藻类、重组蛋白和双链RNA（dsRNA）等各种方案来治疗WSSV。在[具体藻类名称未给出]中dsRNA表达系的开发提高了新载体p2xTRBL和pSS116的效率，使得无需标记物就能筛选转基因细胞。事实证明，生产VP28 dsRNA来对抗对虾中的白斑综合征病毒（WSSV）具有特别的优势。由于在微藻中表达VP28基因的重组质粒的开发，针对WSSV的口服疫苗已被证明非常有效，并且在对虾中显示出很高的存活率。此外，盐生杜氏藻转基因菌株已被改造以产生VP28基因，从而保护小龙虾免受WSSV感染。由于它们对WSSV具有抗病毒特性，诸如[具体藻类名称未给出]种和[具体藻类名称未给出]种等微藻也已被使用，并且可能有助于控制其他水生病毒感染。通过采用可持续和环境友好的做法进行口服疫苗接种，这些微藻成为水产养殖中的一种疾病控制策略，改善了水产养殖农民的财务状况和收入。所有微藻都具有抗病毒特性，并能对抗白斑综合征病毒（WSSV）。
