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柑橘中[具体物质]的表达特征及其相互作用蛋白分析。 （原文中“of”后缺少具体内容）

Expression characteristics of in citrus and analysis of its interacting protein.

作者信息

He Qing, He Xiao

机构信息

School of Medical Technology, Chongqing Three Gorges Medical College, Chongqing, People's Republic of China.

出版信息

Plant Signal Behav. 2025 Dec;20(1):2508418. doi: 10.1080/15592324.2025.2508418. Epub 2025 May 22.

DOI:10.1080/15592324.2025.2508418
PMID:40403246
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12101599/
Abstract

Exploring the resistance genes of citrus to Huanglongbing (HLB) is the foundation and key to citrus disease-resistant breeding. Through the analysis of comparative transcriptome data, we identified six cell wall degradation genes that respond to citrus infection with Las. We selected one of the genes with high differential expression levels and cloned it, naming it . The subcellular localization results of tobacco indicated that the protein is localized in the nucleus, cytoplasm, and cell membrane. Real-time fluorescence quantitative PCR (RT-qPCR) analysis showed that the expression of is related to variety tolerance, tissue location, and symptom development. In addition, we constructed overexpression and silencing vectors for and obtained silencing plants, overexpression and silencing hairy roots, and analyzed the expression characteristics of in response to SA, JA, MeSA and HO induction through RT-qPCR. Using Protein-Protein Interaction (PPI) to predict and screen for a citrus protein CsAGD8 that may interact with CsPG23, and preliminarily verifying its interaction with CsPG23 protein through Yeast Two-hybrid (Y2H). We constructed overexpression and silencing vectors for and obtained overexpression and silencing hairy roots. In summary, it is indicated that CsPG23 may interact with CsAGD8 in response to Las infection.

摘要

探索柑橘对黄龙病（HLB）的抗性基因是柑橘抗病育种的基础和关键。通过对比较转录组数据的分析，我们鉴定出六个响应柑橘感染韧皮部杆菌的细胞壁降解基因。我们选择了其中一个差异表达水平高的基因进行克隆，并将其命名为 。烟草的亚细胞定位结果表明， 蛋白定位于细胞核、细胞质和细胞膜。实时荧光定量PCR（RT-qPCR）分析表明， 的表达与品种耐受性、组织部位和症状发展有关。此外，我们构建了 的过表达和沉默载体，获得了 沉默植株、过表达和沉默毛状根，并通过RT-qPCR分析了 对SA、JA、MeSA和HO诱导的表达特征。利用蛋白质-蛋白质相互作用（PPI）预测并筛选出可能与CsPG23相互作用的柑橘蛋白CsAGD8，并通过酵母双杂交（Y2H）初步验证其与CsPG23蛋白的相互作用。我们构建了 的过表达和沉默载体，获得了 过表达和沉默毛状根。综上所述，表明CsPG23可能在响应韧皮部杆菌感染时与CsAGD8相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/1d8c92465ad7/KPSB_A_2508418_F0010_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/07bba6ff986a/KPSB_A_2508418_F0001_OC.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/f3c5b2649446/KPSB_A_2508418_F0007_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/cb24a0349261/KPSB_A_2508418_F0008_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/2f1040719858/KPSB_A_2508418_F0009_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/1d8c92465ad7/KPSB_A_2508418_F0010_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/07bba6ff986a/KPSB_A_2508418_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/30f1148028da/KPSB_A_2508418_F0002_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/fa3462d56ff6/KPSB_A_2508418_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/2d770a43453e/KPSB_A_2508418_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/3c883b86c861/KPSB_A_2508418_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/3db07ec9b5dc/KPSB_A_2508418_F0006_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/f3c5b2649446/KPSB_A_2508418_F0007_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/cb24a0349261/KPSB_A_2508418_F0008_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/2f1040719858/KPSB_A_2508418_F0009_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/beda/12101599/1d8c92465ad7/KPSB_A_2508418_F0010_OC.jpg

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