Expression characteristics of in citrus and analysis of its interacting protein.

Exploring the resistance genes of citrus to Huanglongbing (HLB) is the foundation and key to citrus disease-resistant breeding. Through the analysis of comparative transcriptome data, we identified six cell wall degradation genes that respond to citrus infection with Las. We selected one of the genes with high differential expression levels and cloned it, naming it . The subcellular localization results of tobacco indicated that the protein is localized in the nucleus, cytoplasm, and cell membrane. Real-time fluorescence quantitative PCR (RT-qPCR) analysis showed that the expression of is related to variety tolerance, tissue location, and symptom development. In addition, we constructed overexpression and silencing vectors for and obtained silencing plants, overexpression and silencing hairy roots, and analyzed the expression characteristics of in response to SA, JA, MeSA and HO induction through RT-qPCR. Using Protein-Protein Interaction (PPI) to predict and screen for a citrus protein CsAGD8 that may interact with CsPG23, and preliminarily verifying its interaction with CsPG23 protein through Yeast Two-hybrid (Y2H). We constructed overexpression and silencing vectors for and obtained overexpression and silencing hairy roots. In summary, it is indicated that CsPG23 may interact with CsAGD8 in response to Las infection.