Cooke Megan E, Riffe Morgan B, Gomes Manuela E, Domingues Rui M A, Burdick Jason A
BioFrontiers Institute, University of Colorado Boulder, Boulder CO 80303, United States of America.
Materials Science & Engineering, University of Colorado Boulder, Boulder CO 80303, United States of America.
Biofabrication. 2025 Jun 3;17(3):033001. doi: 10.1088/1758-5090/addc42.
Suspension bath bioprinting, defined as extrusion bioprinting into a suspension bath consisting of a yield-stress material with fast recovery, emerged over a decade ago. Since this time, many suspension baths have been developed from molecular assemblies to granular media and across a range of synthetic and natural polymers. These suspension baths have been applied to the printing of a wide variety of inks for applications in tissue engineering, fromtissue models to implantable constructs. In a scoping search of published literature over the past decade, 254 articles were identified that met various definitions related to suspension baths for biofabrication in order to gain a perspective on the various materials used and their applications; however, the literature is much more broad than this due to the disperse terminology that has been applied to the approach. This article gives a perspective on the progress that has been made in suspension bath printing, including applications of the technology and challenges that exist across the field, as well as provides a look to the future of where such printing methods will make an impact.
悬浮浴生物打印，定义为将生物材料挤压打印到由具有快速恢复能力的屈服应力材料组成的悬浮浴中，这项技术在十多年前就已出现。从那时起，许多悬浮浴已从分子组装体发展到颗粒介质，并涵盖了一系列合成和天然聚合物。这些悬浮浴已应用于打印各种用于组织工程的墨水，从组织模型到可植入构建体。在对过去十年发表的文献进行的范围搜索中，共识别出254篇文章，这些文章符合与用于生物制造的悬浮浴相关的各种定义，以便了解所使用的各种材料及其应用；然而，由于应用于该方法的术语分散，文献范围比这要广泛得多。本文阐述了悬浮浴打印所取得的进展，包括该技术的应用以及该领域存在的挑战，并展望了此类打印方法将产生影响的未来方向。
