Zhang Xian, Shan Baolong, Li Yingdong
College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, China.
College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, China.
Gene. 2025 Aug 20;962:149579. doi: 10.1016/j.gene.2025.149579. Epub 2025 May 20.
Dybowski's frog (Rana dybowskii) is an important species in northeastern China, with significant ecological and economic value. Understanding the molecular mechanisms underlying gonadal differentiation is crucial for improving aquaculture practices and conserving biodiversity. This study investigated the transcriptomic profiles of male and female R. dybowskii gonads to identify sex-specific molecular markers and pathways involved in sexual differentiation. Using RNA sequencing, we comprehensively analyzed the gene expression profiles of gonadal tissues from both sexes at different developmental stages. Our findings revealed significant differences in the expression of key genes related to gonadal development, steroidogenesis, and sex hormone signaling. Additionally, we identified several novel genes that may play roles in the regulation of gonadal differentiation. These molecular insights provide a better understanding of the reproductive biology of R. dybowskii and offer potential targets for improving the management of aquaculture breeding programs. These results also contribute to a broader understanding of gonadal differentiation in amphibians, with implications for conservation and sustainable farming practices.
东北林蛙（Rana dybowskii）是中国东北地区的重要物种，具有重要的生态和经济价值。了解性腺分化的分子机制对于改进水产养殖实践和保护生物多样性至关重要。本研究调查了雄性和雌性东北林蛙性腺的转录组概况，以确定参与性别分化的性别特异性分子标记和途径。通过RNA测序，我们全面分析了不同发育阶段两性性腺组织的基因表达谱。我们的研究结果揭示了与性腺发育、类固醇生成和性激素信号传导相关的关键基因表达存在显著差异。此外，我们还鉴定了几个可能在性腺分化调控中发挥作用的新基因。这些分子见解有助于更好地理解东北林蛙的生殖生物学，并为改进水产养殖育种计划的管理提供潜在靶点。这些结果也有助于更广泛地了解两栖动物的性腺分化，对保护和可持续养殖实践具有重要意义。
