Xue Haotian, Kan Xianwen
Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-Based Materials, Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China.
Analyst. 2025 Jun 23;150(13):2792-2799. doi: 10.1039/d5an00410a.
The development of integrated dual signal outputs for the reliable and accurate determination of glutathione (GSH) is highly significant for its key role in physiological processes. Herein, a colorimetric/photoelectrochemical (PEC) dual-mode sensing platform was constructed based on the GSH-triggered decomposition of a copper-organic framework (CuHPT). A hollow nanostructured type-II heterojunction of CoS/In-CdS was synthesized using an imidazolate framework (ZIF-67) as a template. CuHPT was decomposed in the presence of GSH to form catechol ligands and Cu, enabling dual-mode sensing. An ion exchange reaction between the produced Cu and Cd of CoS/In-CdS resulted in the formation of CuS, causing a decrease in the photocurrent and the sensitive detection of GSH. Cu catalyzed HO to produce ˙OH a Fenton-like reaction, achieving colorimetric sensing. The proposed dual-mode sensor based on the target-triggered decomposition of CuHPT exhibited linear responses for colorimetric and PEC sensing of GSH in the range of 0.05-1.20 mM and 0.5-800 μM with the limit of detections (LODs) of 18 μM and 0.11 μM, respectively. The reliable determination of GSH in human serum provides a new possibility for the application of the present dual-mode sensor in clinical assays.
开发用于可靠且准确测定谷胱甘肽（GSH）的集成双信号输出对于其在生理过程中的关键作用具有重要意义。在此，基于谷胱甘肽触发的铜有机框架（CuHPT）分解构建了一种比色/光电化学（PEC）双模式传感平台。以咪唑框架（ZIF - 67）为模板合成了CoS/In - CdS的中空纳米结构II型异质结。CuHPT在谷胱甘肽存在下分解形成儿茶酚配体和铜，实现双模式传感。CoS/In - CdS中产生的铜与镉之间的离子交换反应导致形成CuS，引起光电流降低并实现对谷胱甘肽的灵敏检测。铜催化H₂O产生˙OH，这是一种类芬顿反应，实现比色传感。基于CuHPT的目标触发分解所提出的双模式传感器对谷胱甘肽的比色和PEC传感在0.05 - 1.20 mM和0.5 - 800 μM范围内呈现线性响应，检测限（LOD）分别为18 μM和0.11 μM。在人血清中可靠测定谷胱甘肽为当前双模式传感器在临床检测中的应用提供了新的可能性。
