Liu Zheng, Feng Yufan, Cui Yongxi, Wang Yumeng, Fu Zhuoyan, Zhao Jianwei, Li Hongxia, Sun Chunyan
Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun 130062, PR China.
Shenzhen HUASUAN Technology Co., Ltd., Shenzhen 518107, PR China.
Food Chem. 2025 Oct 1;488:144892. doi: 10.1016/j.foodchem.2025.144892. Epub 2025 May 23.
Nanozymes exhibit huge potential in developing sensors, there are still significant challenges in designing nanozymes and exploring catalytic mechanisms. Inspired by the ligand-environment effect, ligands with differently charged substituents were introduced into a copper metal-organic framework (Cu-BDC-X, X = OH, NH, COOH, NO) to study the relationship between ligand structure and laccase-like activity. Attractively, tuning the ligand substituents directly regulates the laccase-like activity of Cu-BDC. In-depth experiments showed that the introduction of electron-withdrawing substituent ligands not only increased the ratio of Cu in Cu-BDC but also enlarged the pore diameter of the Cu-BDC. As a concept of the application, a Cu-BDC-NO nanozyme-based colorimetric sensor was developed for the detection of glyphosate pesticide. Importantly, Cu-BDC-NO nanozyme was embedded in hydrogels to fabricate a portable platform for in situ detection of glyphosate pesticide. This work is expected to provide new ideas for building advanced nanozymes and holds great promise for food safety.
纳米酶在开发传感器方面具有巨大潜力，但在设计纳米酶和探索催化机制方面仍存在重大挑战。受配体-环境效应的启发，将带有不同电荷取代基的配体引入到铜金属有机框架（Cu-BDC-X，X = OH、NH、COOH、NO）中，以研究配体结构与漆酶样活性之间的关系。引人注目的是，调节配体取代基可直接调节Cu-BDC的漆酶样活性。深入实验表明，引入吸电子取代基配体不仅增加了Cu-BDC中Cu的比例，还扩大了Cu-BDC的孔径。作为一种应用概念，开发了一种基于Cu-BDC-NO纳米酶的比色传感器用于检测草甘膦农药。重要的是，将Cu-BDC-NO纳米酶嵌入水凝胶中，制造了一个用于原位检测草甘膦农药的便携式平台。这项工作有望为构建先进的纳米酶提供新思路，并在食品安全方面具有巨大潜力。
