草本枝孢菌的高效液相色谱法。用免疫技术鉴定过敏原。

High-performance liquid chromatography of Cladosporium herbarum. Identification of allergens with immunological techniques.

作者信息

Landmark E, Aukrust L

出版信息

Int Arch Allergy Appl Immunol. 1985;78(1):71-6. doi: 10.1159/000233865.

Abstract

High-performance liquid chromatography (HPLC) using a gel permeation column was applied to separate components in a crude extract of the mould Cladosporium herbarum. Allergenic activity as well as individual allergens in collected fractions were analyzed by direct radioallergosorbent test (RAST) and fused rocked radioimmunoelectrophoresis (FRRIE), respectively. Thus, information of the molecular weight as well as the importance of the individual allergens were obtained simultaneously in a system showing a high degree of resolution. The study demonstrated that the crude extract of C. herbarum contained allergens mainly in the molecular weight range between 10,000 and 300,000 daltons. The results demonstrated the importance of carrying out both RAST and FRRIE to detect all allergens.

摘要

采用凝胶渗透柱的高效液相色谱法（HPLC）用于分离草本枝孢霉菌粗提物中的成分。分别通过直接放射变应原吸附试验（RAST）和融合火箭放射免疫电泳（FRRIE）分析收集组分中的致敏活性以及单个变应原。因此，在具有高分辨率的系统中同时获得了分子量信息以及单个变应原的重要性。该研究表明，草本枝孢霉粗提物中的变应原主要在分子量10,000至300,000道尔顿范围内。结果表明，同时进行RAST和FRRIE检测所有变应原的重要性。