通过定量免疫电泳方法对草本枝孢提取物进行表征，特别关注速发型过敏反应。

Cladosporium herbarum extract characterized by means of quantitative immunoelectrophoretic methods with special attention to immediate type allergy.

作者信息

Lowenstein H, Aukrust L, Gravesen S

出版信息

Int Arch Allergy Appl Immunol. 1977;55(1-6):1-12. doi: 10.1159/000231900.

DOI:10.1159/000231900

Abstract

Freeze-dried extract of Cladosporium herbarum Link ex Fr. was obtained by growing, harvesting, extracting, centrifuging, dialysing and freeze-drying. Quantitative immunoelectrophoresis using rabbit antibodies revealed the extraction procedure to be reproducible and the extract to be composed of 57 antigens, none of which originated from the substrate used in the growth. The molecular weight distribution and the approximate molecular weight of some antigens of C. herbarum were obtained using gel filtration. The pI distribution and the approximate pIs of a few distinct antigens of C. herbarum were obtained by isoelectric focusing. Preliminary identification of 4 allergens from C. herbarum was performed by means of CRIE (crossed radioimmunoelectrophoresis).

摘要

通过培养、收获、提取、离心、透析和冷冻干燥获得了匐柄霉（Cladosporium herbarum Link ex Fr.）的冻干提取物。使用兔抗体进行的定量免疫电泳显示提取过程具有可重复性，提取物由57种抗原组成，其中没有一种源自生长所用的底物。通过凝胶过滤获得了匐柄霉某些抗原的分子量分布和近似分子量。通过等电聚焦获得了匐柄霉一些不同抗原的pI分布和近似pI值。通过交叉放射免疫电泳（CRIE）对匐柄霉的4种过敏原进行了初步鉴定。