Wang Yijie, Liu Yujia, Du Chunjing, Zhang Yue, Zhu Liuluan
Beijing Key Laboratory of Viral Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, China.
Beijing Institute of Infectious Diseases, Beijing, China.
Sci Data. 2025 May 23;12(1):853. doi: 10.1038/s41597-025-05181-8.
Neutrophils, the most abundant white blood cells in human circulation, play a crucial role in innate immunity. One of their key defense mechanisms is the formation of neutrophil extracellular traps (NETs), web-like structures composed of chromatin and antimicrobial proteins that help capture and neutralize pathogens. While previous studies have identified a limited set of NET-associated proteins, the comprehensive proteomic landscape of NETs induced by different stimuli remains largely unexplored. In this study, we used data-independent acquisition mass spectrometry to analyze the proteomic composition of NETs induced by five distinct stimuli: β-glucan, lipopolysaccharide, polyinosinic-polycytidylic acid sodium, resiquimod, and severe fever with thrombocytopenia syndrome bunyavirus. Across all conditions, we identified 5,868 NET-associated proteins, revealing significant stimulus-dependent differences in protein composition. Notably, differentially expressed proteins were detected in each condition, highlighting unique proteomic signatures that may reflect distinct immune responses. This dataset offers key insights into the proteomic diversity of NETs and their role in immune regulation, providing a foundation for future research on NET-mediated immunity in infectious and inflammatory diseases.
中性粒细胞是人体循环中数量最多的白细胞，在固有免疫中发挥着关键作用。它们的关键防御机制之一是形成中性粒细胞胞外陷阱（NETs），这是一种由染色质和抗菌蛋白组成的网状结构，有助于捕获和中和病原体。虽然先前的研究已经确定了一组有限的与NET相关的蛋白质，但不同刺激诱导产生的NETs的全面蛋白质组学图谱在很大程度上仍未被探索。在这项研究中，我们使用数据非依赖型采集质谱法分析了由五种不同刺激诱导产生的NETs的蛋白质组组成：β-葡聚糖、脂多糖、聚肌苷酸-聚胞苷酸钠、瑞喹莫德和严重发热伴血小板减少综合征布尼亚病毒。在所有条件下，我们鉴定出了5868种与NET相关的蛋白质，揭示了蛋白质组成上显著的刺激依赖性差异。值得注意的是，在每种条件下都检测到了差异表达的蛋白质，突出了可能反映不同免疫反应的独特蛋白质组学特征。该数据集为NETs的蛋白质组学多样性及其在免疫调节中的作用提供了关键见解，为未来关于NET介导的感染性和炎症性疾病免疫的研究奠定了基础。
