Najm Mazin A A, Shakir Hussein A, Hasen Sabah T, Jawad Kareem H, Hasoon Buthenia A, Jabir Majid S, Issa Ali A, Albukhaty Salim, Gatasheh Mansour K, Molla Mohammad H
Department of Pharmacy, Mazaya University College, Thi-Qar, Iraq.
Laser & Optoelectronics Engineering Department, University of Technology, Baghdad, Iraq.
Sci Rep. 2025 May 23;15(1):18014. doi: 10.1038/s41598-025-93569-2.
The increasing of multi-drug among pathogenic microbes is rendering antibiotics ineffective. Consequently, efforts are now concentrated on addressing this challenge through developing novel antibiotics and enhancing existing ones. This study combined ciprofloxacin (CIP) with titanium dioxide nanoparticles (TiONPs). We characterized the prepared nanoparticles (NPs) using several methods, including UV-Vis spectra, XRD, FESEM, TEM, and FTIR. The well diffusion agar was used to study the antibacterial activity of ciprofloxacin (CIP) alone and combined with titanium dioxide nanoparticles CIP@TiONPs. CIP@TiONPs showed higher antibacterial activity against Klebsiella pneumoniae (K.pneumoniae) and Streptococcus mutans S.mutans. The CIP@TiONPs showed remarkable inhibitory properties compared to CIP alone and TiONP alone, with its inhibition zone 28.50 ± 0.20 and 17.50 ± 0.10 in K.pneumoniae and S.mutans, respectively. Insilico study was done on bacterial strains to describe the effective binding behavior towards the ciprofloxacin@TiO adsorption system. The best conformers, from 50 conformational adsorption systems, were analyzed with a significant favorable inhibition with binding energy values of -9.61 kcal/mol and - 9.40 kcal/mol with K.pneumoniae and S. mutans, respectively. The interaction between CIP@TiONPs nanoparticles and Klebsiella pneumonia (ID: 8JGW) was studied using 50 conformations. The results showed binding energies up to -9.61 kcal/mol, indicating high interaction efficacy. Compared to TiONPs and CIP alone, CIP@TiONPs displayed the highest antibacterial and anti-biofilm properties against pathogenic bacteria. CIP@TiONPs have demonstrated promising results, suggesting that they may prove to be a dependable treatment for K. pneumoniae and S.mutans in the future and a possible agent for reducing bacterial biofilm during bacterial infections.
致病微生物中多重耐药性的增加正使抗生素失效。因此，目前的努力集中在通过开发新型抗生素和改进现有抗生素来应对这一挑战。本研究将环丙沙星（CIP）与二氧化钛纳米颗粒（TiONPs）相结合。我们使用多种方法对制备的纳米颗粒（NPs）进行了表征，包括紫外可见光谱、X射线衍射、场发射扫描电子显微镜、透射电子显微镜和傅里叶变换红外光谱。采用琼脂扩散法研究了单独的环丙沙星（CIP）以及与二氧化钛纳米颗粒结合的环丙沙星（CIP@TiONPs）的抗菌活性。CIP@TiONPs对肺炎克雷伯菌（K.pneumoniae）和变形链球菌（S.mutans）表现出更高的抗菌活性。与单独的CIP和单独的TiONP相比，CIP@TiONPs表现出显著的抑制特性，其在肺炎克雷伯菌和变形链球菌中的抑菌圈分别为28.50±0.20和17.50±0.10。对细菌菌株进行了计算机模拟研究，以描述对环丙沙星@TiO吸附系统的有效结合行为。从50个构象吸附系统中筛选出的最佳构象体进行了分析，其对肺炎克雷伯菌和变形链球菌的结合能值分别为-9.61 kcal/mol和-9.40 kcal/mol，具有显著的有利抑制作用。使用50种构象研究了CIP@TiONPs纳米颗粒与肺炎克雷伯菌（ID：8JGW）之间的相互作用。结果显示结合能高达-9.61 kcal/mol，表明相互作用效率高。与单独的TiONPs和CIP相比，CIP@TiONPs对病原菌表现出最高的抗菌和抗生物膜特性。CIP@TiONPs已显示出有前景的结果，表明它们未来可能被证明是治疗肺炎克雷伯菌和变形链球菌的可靠药物，并且可能是减少细菌感染期间细菌生物膜的一种药物。
