Gorzolka Karin, Böttcher Christoph, Thieme Thomas, Gäbelein Roman, Thieme Ramona, Meiners Torsten
Institute for Ecological Chemistry, Plant Analysis and Stored Product Protection, Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Königin-Luise-Straße 19, 14195 Berlin, Germany.
BTL Bio-Test Labor GmbH Sagerheide, Thünenplatz 1, 18190 Sanitz, Germany.
J Agric Food Chem. 2025 Jun 4;73(22):13555-13567. doi: 10.1021/acs.jafc.5c01297. Epub 2025 May 25.
The fatty acid-solamine conjugate C16:3(7,10,13)-solamine was isolated from leaves of the wild potato species and structurally characterized using spectroscopic methods. The exogenous application of C16:3(7,10,13)-solamine reduced the feeding of Colorado potato beetles () and potato flea beetles () on leaf disks and inhibited the germination of sporangia produced by the oomycete pathogen and subsequent plant infection. The use of liquid chromatography-coupled tandem mass spectrometry enabled the detection and characterization of numerous acyl solamines in leaves and in the belowground organs of numerous accessions of eight wild potato species and of cultivars of . The annotated acyl solamines comprise medium- and long-chain fatty acyl solamines, whose acyl groups differ in chain length, the number of double bond equivalents, and the number of oxygenations, as well as hydroxycinnamoyl solamines. The profiles of the detected acyl solamines exhibited notable variation between different species, as well as between different tissues within a species. The potential role of acyl solamines in plant defense and their potential utility in plant protection and resistance breeding are discussed.
从野生马铃薯品种的叶片中分离出脂肪酸 - 乙醇胺共轭物C16:3(7,10,13)-乙醇胺，并采用光谱方法对其进行结构表征。外源施用C16:3(7,10,13)-乙醇胺减少了科罗拉多马铃薯甲虫和马铃薯跳甲对叶片圆片的取食，并抑制了卵菌病原体产生的孢子囊的萌发以及随后的植物感染。利用液相色谱 - 串联质谱法能够检测和表征8种野生马铃薯品种的多个种质以及栽培品种的叶片和地下器官中的多种酰基乙醇胺。注释的酰基乙醇胺包括中链和长链脂肪酰基乙醇胺，其酰基在链长、双键当量数和氧化数方面存在差异，以及羟基肉桂酰基乙醇胺。检测到的酰基乙醇胺谱在不同的马铃薯品种之间以及同一品种的不同组织之间表现出显著差异。讨论了酰基乙醇胺在植物防御中的潜在作用及其在植物保护和抗性育种中的潜在用途。
