Aydogdu Ayfer Burcu, Olmez Aysegul, Akca Gulcin
Department of Pediatric Dentistry, Gazi University Faculty of Dentistry, Ankara, Cankaya, Turkey.
Department of Medical Microbiology, Gazi University Faculty of Dentistry, Ankara, Cankaya, Turkey.
Int J Clin Pediatr Dent. 2025 Mar;18(3):245-250. doi: 10.5005/jp-journals-10005-3067. Epub 2025 Apr 19.
This study aims to compare the efficiency of caries removal between the chemomechanical caries removal agent Brix 3000 and the conventional method using a laser fluorescent device (DIAGNOdent Pen), caries detection dye, microbial culture, and real-time polymerase chain reaction (PCR) methods.
The study involved 64 primary molars with dentinal caries between March and June 2022. Standardization was achieved based on International Caries Detection and Assessment System (ICDAS-II) criteria (score 6) and DIAGNOdent Pen measurements (30 and above). Caries were removed using the conventional method (32) and Brix 3000 (32). Following this, dentin samples were collected, and measurements were made with the DIAGNOdent Pen to evaluate the presence of residual caries in both groups. After the measurements, the presence of residual caries was evaluated in both groups using caries detection dye. The decrease in values obtained by microbial culture and real-time PCR methods of dentin samples taken before and after caries removal was evaluated.
When DIAGNOdent Pen and caries detection dye were evaluated, the caries removal efficiency of the conventional method was found to be higher ( < 0.05). As a result of the microbial culture evaluation for and total bacteria, the caries removal efficiencies of both methods were found to be similar ( > 0.05). The caries removal efficiency of Brix 3000 was found to be higher as a result of the evaluation with real-time PCR for ( < 0.05).
It is thought that Brix 3000 is an effective agent for caries removal and may be an alternative agent to the conventional method, especially considering its advantages in children.
Aydogdu AB, Olmez A, Akca G. Chemomechanical Caries Removal with Enzymatic Technology: A Comparative Study. Int J Clin Pediatr Dent 2025;18(3):245-250.
本研究旨在比较化学机械去龋剂Brix 3000与使用激光荧光装置（DIAGNOdent Pen）、龋病检测染料、微生物培养和实时聚合酶链反应（PCR）方法的传统方法在去除龋坏组织方面的效率。
本研究纳入了2022年3月至6月间64颗患有牙本质龋的乳牙。根据国际龋病检测与评估系统（ICDAS-II）标准（评分6）和DIAGNOdent Pen测量值（30及以上）实现标准化。使用传统方法（32颗）和Brix 3000（32颗）去除龋坏组织。之后，收集牙本质样本，并用DIAGNOdent Pen进行测量，以评估两组中残留龋的存在情况。测量后，使用龋病检测染料评估两组中残留龋的存在情况。评估龋坏组织去除前后采集的牙本质样本通过微生物培养和实时PCR方法获得的值的下降情况。
在评估DIAGNOdent Pen和龋病检测染料时，发现传统方法的去龋效率更高（P<0.05）。对变形链球菌和总细菌进行微生物培养评估的结果显示，两种方法的去龋效率相似（P>0.05）。通过对变形链球菌进行实时PCR评估，发现Brix 3000的去龋效率更高（P<0.05）。
认为Brix 3000是一种有效的去龋剂，尤其是考虑到其在儿童中的优势，它可能是传统方法的替代剂。
Aydogdu AB, Olmez A, Akca G. 酶技术化学机械去龋：一项比较研究。《国际临床儿科牙科学杂志》2025；18(3):245 - 250。
