Zhu Wen, Han Shuo, Cheng Yao, Yu Zhihong, Zhao Guozhu, He Xiangwei
College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, China.
Front Microbiol. 2025 May 9;16:1585700. doi: 10.3389/fmicb.2025.1585700. eCollection 2025.
Phytoremediation offers a promising strategy for addressing the global challenge of land desertification. In the Mu Us Sandy Land of China, Krasch. has emerged as a key species for desertification control. Its root-associated microbial communities may enhance the plant's adaptability to sandy, nutrient-poor environments. Despite their ecological significance, comprehensive investigations of these microbial communities remain limited. In this study, microbial communities in the root zone (i.e., rhizosphere soil, non-rhizosphere soil, and root endosphere) of were analyzed via high-throughput sequencing and different isolation approaches across successional stages (moving dunes, semi-fixed dunes, and fixed dunes) in the Mu Us Sandy Land of northern China. Metagenomic analysis revealed that microbial diversity was significantly higher in the rhizosphere and non-rhizosphere soils than in the root endosphere; moving dunes exhibited lower diversity than semi-fixed and fixed dunes. Meanwhile, distinct microbial community structures across successional stages were revealed by principal coordinates analysis (PCoA), demonstrating substantial differences between the root endosphere and other zones. Environmental factors, including nitrate nitrogen (NO -N), organic matter (OM), available potassium (AK), and total potassium (TK), significantly influenced microbial community composition. Moreover, dominant genera such as and were identified, potentially contributing to growth. From a culturomics perspective, 93 bacterial isolates were obtained using conventional streak plate and colony pick methods, with Firmicutes (37.63%) and (23.66%) identified as the dominant taxa. In parallel, 14 fungal strains were isolated, primarily belonging to (35.71%) and (21.43%), both of which are well-documented for their stress tolerance in arid ecosystems. A high-throughput cultivation and identification method, tailored to recover rare and slow-growing bacteria, was employed and successfully broadened the cultured diversity to include Proteobacteria (46.43%) and representatives of the rarely cultivated Deinococcus-Thermus phylum. This study provides metagenomic with culturomics insights into the microbial communities associated with , enhancing the understanding of plant-microbe interactions in sandy land ecosystems.
植物修复为应对全球土地沙漠化挑战提供了一种很有前景的策略。在中国毛乌素沙地，柠条锦鸡儿已成为荒漠化治理的关键物种。其根系相关微生物群落可能会增强植物对沙质、营养贫瘠环境的适应性。尽管这些微生物群落具有生态意义，但对它们的全面研究仍然有限。在本研究中，通过高通量测序和不同的分离方法，对中国北方毛乌素沙地不同演替阶段（流动沙丘、半固定沙丘和固定沙丘）柠条锦鸡儿根区（即根际土壤、非根际土壤和根内圈）的微生物群落进行了分析。宏基因组分析表明，根际和非根际土壤中的微生物多样性显著高于根内圈；流动沙丘的多样性低于半固定沙丘和固定沙丘。同时，主坐标分析（PCoA）揭示了不同演替阶段独特的微生物群落结构，表明根内圈与其他区域之间存在显著差异。环境因素，包括硝态氮（NO₃-N）、有机质（OM）、速效钾（AK）和全钾（TK），显著影响微生物群落组成。此外，还鉴定出了诸如假单胞菌属和芽孢杆菌属等优势属，它们可能对柠条锦鸡儿的生长有促进作用。从培养组学的角度来看，使用传统的划线平板法和菌落挑选法获得了93株细菌分离株，其中厚壁菌门（37.63%）和芽孢杆菌属（23.66%）被确定为优势分类群。同时，分离出了14株真菌菌株，主要属于曲霉属（35.71%）和青霉属（21.43%），这两个属在干旱生态系统中的抗逆性都有充分的文献记载。采用了一种针对回收稀有和生长缓慢细菌的高通量培养和鉴定方法，并成功扩大了培养的多样性，包括变形菌门（46.43%）和很少培养的嗜热放线菌门的代表。本研究为与柠条锦鸡儿相关的微生物群落提供了宏基因组和培养组学见解，增进了对沙地生态系统中植物-微生物相互作用的理解。
