Yovchevska Lyudmila, Miteva-Staleva Jeny, Dishliyska Vladislava, Stoyancheva Galina, Gocheva Yana, Abrashev Radoslav, Spasova Boryana, Angelova Maria, Krumova Ekaterina
Departament of Mycology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev str. bl.26, 1113 Sofia, Bulgaria.
Departament of General Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev str. bl.26, 1113 Sofia, Bulgaria.
Molecules. 2025 Mar 7;30(6):1196. doi: 10.3390/molecules30061196.
In recent years, there has been increasing interest in the study of extremophilic microorganisms, which include halophiles and halotolerants. These microorganisms, able to survive and thrive optimally in a wide range of environmental extremes, are polyextremophiles. In this context, one of the main reasons for studying them is to understand their adaptative mechanisms to stress caused by extreme living conditions. In this paper, a fungal strain P13, isolated from saline soils around Pomorie Lake, Bulgaria, was used. The effect of elevated concentrations of sodium chloride on the growth and morphology as well as on the physiology of the model strain was investigated. P13 demonstrated high tolerance to NaCl, showing remarkable growth in liquid and agar media. In order to establish the relationship between salt- and oxidative stress, changes in the cell biomarkers of oxidative stress, such as oxidatively damaged proteins, lipid peroxidation, and levels of reserve carbohydrates of the studied strain were evaluated. The involvement of antioxidant enzyme defense in the adaptive strategy of the halotolerant strain against elevated NaCl concentrations was investigated.
近年来，人们对嗜极端微生物的研究兴趣日益浓厚，嗜极端微生物包括嗜盐菌和耐盐菌。这些微生物能够在各种极端环境中生存并最佳地生长繁殖，属于多嗜极端菌。在这种情况下，研究它们的主要原因之一是了解其对极端生存条件所造成压力的适应机制。本文使用了从保加利亚波莫瑞湖周边盐渍土中分离出的一株真菌菌株P13。研究了高浓度氯化钠对该模式菌株生长、形态以及生理的影响。P13对氯化钠表现出高耐受性，在液体和琼脂培养基中均有显著生长。为了建立盐胁迫与氧化应激之间的关系，评估了所研究菌株的氧化应激细胞生物标志物的变化，如氧化损伤蛋白、脂质过氧化和储备碳水化合物水平。研究了抗氧化酶防御在耐盐菌株应对高浓度氯化钠的适应性策略中的作用。
