中国福州胎膜早破孕妇中B族分离株的分子流行病学研究
Molecular Epidemiology of Group B Isolates from Pregnant Women with Premature Rupture of Membranes in Fuzhou, China.
作者信息
Liang Bin, Chen Huiyu, Yu Donghong, Zhao Wantong, Cai Xiaoling, Qiu Huahong, Xu Liangpu
机构信息
Medical Genetic Diagnosis and Therapy Center, Fujian Key Laboratory for Prenatal Diagnosis and Birth Defect, Fujian Maternity and Child Health Hospital College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, People's Republic of China.
Laboratory Department, Fujian Maternity and Child Health Hospital College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, People's Republic of China.
出版信息
Infect Drug Resist. 2023 Jan 14;16:269-278. doi: 10.2147/IDR.S393935. eCollection 2023.
OBJECTIVE
This study investigated the molecular epidemiology of Group B (GBS) in pregnant women with premature rupture of membranes (PROM) in Fuzhou region of China as a source of clinical reference.
METHODS
GBS isolates were obtained from pregnant women with PROM. All isolates were genotyped, serotyped, and tested for drug-resistance and virulence genes using PCR and DNA sequencing. Antibiotic susceptibility testing was performed using the Vitek 2 automated system.
RESULTS
Among the 140 GBS isolates, seventeen sequence types (STs) were identified, of which ST19 (20.0%) was the most prevalent, followed by ST862, ST10, and ST12. Three clonal complexes (CC19, CC10 and CC1) were identified. The predominant serotype was III (45.7%), followed by V (23.6%), Ib (18.6%), Ia (7.1%), and II (3.6%). The prevalence of multidrug resistance was 72.8% (102/140). All isolates were susceptible to penicillin G, ampicillin, quinupristin, linezolid, vancomycin, and tigecycline. The majority of isolates were resistant to erythromycin (70.0%), clindamycin (72.1%), and tetracycline (81.4%), and 28.6% of isolates were resistant to levofloxacin and moxifloxacin. Of the 98 erythromycin-resistant strains, , and were detected in 100%, 70.4%, 49.0%, 22.4%, 13.3%, and 9.2%, respectively. No was detected among 101 clindamycin-resistant strains. Of the 114 tetracycline-resistant strains, and were detected in 52.6%, 61.4%, 7.9%, and 23.7%, respectively. Regarding virulence genes, all strains carried and , followed by (98.6%), and (80.7%), whereas only one strain carried .
CONCLUSION
ST19/III and ST862/III were the most prevalent GBS subtypes. Penicillin G remains a first-line antibiotic for intrapartum antibiotic prophylaxis and treatment of GBS infections. The prevalence of resistance to clindamycin, erythromycin, and tetracycline is high among GBS isolates in the Fuzhou region. ST862 and ST651 are emerging animal origin STs in human infections, and may become potential zoonotic threats.
目的
本研究调查中国福州地区胎膜早破（PROM）孕妇中B族链球菌（GBS）的分子流行病学，为临床提供参考依据。
方法
从胎膜早破孕妇中分离GBS菌株。所有分离株进行基因分型、血清分型，并采用聚合酶链反应（PCR）和DNA测序检测耐药基因和毒力基因。使用Vitek 2自动化系统进行药敏试验。
结果
在140株GBS分离株中，鉴定出17种序列型（STs），其中ST19（20.0%）最为常见，其次是ST862、ST10和ST12。鉴定出三个克隆复合体（CC19、CC10和CC1）。主要血清型为III型（45.7%），其次是V型（23.6%）、Ib型（18.6%）、Ia型（7.1%）和II型（3.6%）。多重耐药率为72.8%（102/140）。所有分离株对青霉素G、氨苄西林、奎奴普丁、利奈唑胺、万古霉素和替加环素敏感。大多数分离株对红霉素（70.0%）、克林霉素（72.1%）和四环素（81.4%）耐药，28.6%的分离株对左氧氟沙星和莫西沙星耐药。在98株红霉素耐药菌株中，分别有100%、70.4%、49.0%、22.4%、13.3%和9.2%检测到……。在101株克林霉素耐药菌株中未检测到……。在114株四环素耐药菌株中，分别有52.6%、61.4%、7.9%和23.7%检测到……。关于毒力基因，所有菌株均携带……和……，其次是……（98.6%）和……（80.7%），而只有一株携带……。
结论
ST19/III和ST862/III是最常见的GBS亚型。青霉素G仍然是产时抗生素预防和治疗GBS感染的一线抗生素。福州地区GBS分离株对克林霉素、红霉素和四环素的耐药率较高。ST862和ST651是人类感染中新出现的动物源性STs，可能成为潜在的人畜共患病威胁。
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