Wang Yingjie, Shen Qingqing, Yan Ruxu, Wang Meng, Xu Min, Chen Hanxiang, Li Dong
Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Medicine and Health Key Laboratory of Laboratory Medicine, Jinan, Shandong, People's Republic of China.
School of Public Health and Health Management, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, People's Republic of China.
J Inflamm Res. 2025 May 21;18:6439-6451. doi: 10.2147/JIR.S524188. eCollection 2025.
Neonatal respiratory distress syndrome (NRDS) is the leading cause of neonatal death. Changes in plasma immunoglobulin G (IgG) N-glycosylation have been demonstrated in a variety of diseases. However, its implications and clinical significance in NRDS remain to be clarified.
To determine the effect of IgG N-glycosylation on NRDS, we recruited 88 NRDS participants and 120 control participants from December 2021 to September 2022. Plasma was collected, IgG was isolated and purified, and the glycogram was analyzed by ultra performance liquid chromatography (UPLC) with fluorescence detector.
The occurrence of premature rupture of membranes (PROM) [OR=9.043(1.036-78.966), P=0.046] and the elevation of γ-glutamyltransferase (GGT) [OR=1.015(1.001-1.029), P=0.032] were independent risk factors for the occurrence of NRDS. Furthermore, the area percentages of GP1, GP3, GP4, GP11, GP13, and GP24 were significantly higher in NRDS patients compared with control group. Conversely, GP14 was observed to be significantly lower. Furthermore, an increase in plasma IgG sialylation and core fucosylation was observed in NRDS, whereas the modification with galactosylation was decreased. The model constructed using GP1, GP13, GP14, PROM, and GGT as composite indices demonstrated robust predictive performance (AUC=0.902, 95% CI: 0.851-0.953).
Patients with NRDS frequently exhibit alterations in the glycosylation of plasma IgG. These findings provide new insights into the diagnosis of NRDS and clinical treatment.
新生儿呼吸窘迫综合征（NRDS）是新生儿死亡的主要原因。血浆免疫球蛋白G（IgG）N-糖基化的变化已在多种疾病中得到证实。然而，其在NRDS中的意义和临床重要性仍有待阐明。
为确定IgG N-糖基化对NRDS的影响，我们在2021年12月至2022年9月招募了88名NRDS参与者和120名对照参与者。收集血浆，分离并纯化IgG，采用超高效液相色谱（UPLC）结合荧光检测器分析糖谱。
胎膜早破（PROM）的发生[比值比（OR）=9.043（1.036-78.966），P=0.046]和γ-谷氨酰转移酶（GGT）升高[OR=1.015（1.001-1.029），P=0.032]是NRDS发生的独立危险因素。此外，与对照组相比，NRDS患者中GP1、GP3、GP4、GP11、GP13和GP24的面积百分比显著更高。相反，观察到GP14显著更低。此外，在NRDS中观察到血浆IgG唾液酸化和核心岩藻糖基化增加，而半乳糖基化修饰减少。以GP1、GP13、GP14、PROM和GGT作为综合指标构建的模型显示出强大的预测性能（曲线下面积[AUC]=0.902，95%置信区间[CI]：0.851-0.953）。
NRDS患者血浆IgG糖基化经常出现改变。这些发现为NRDS的诊断和临床治疗提供了新的见解。
