Jaya A, Josh F, Prihantono P, Laidding S R, Sulmiati S, Soekamto T H, Zainuddin A A
Division of Plastic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
Division of Plastic and Reconstructive Surgery, Department of Surgery, Dr. Wahidin Sudirohusodo Hospital, Makassar, Indonesia.
Ann Burns Fire Disasters. 2025 Mar 31;38(1):17-24. eCollection 2025 Mar.
Experimental studies have shown that the effects of stromal vascular fractions and platelet-rich plasma on deep dermal burn healing were encouraging. By measuring the blood level of platelet-derived growth factor (PDGF)-BB, we evaluated their effect on full-thickness burns in this study. Forty-eight Wistar rats were separated into four main groups: group A rats were administered combined SVF and PRP injections, group B rats were topically administered Vaseline, group C rats were administered placebo injections, and group D rats were without burns. Depending on the day of euthanasia, these groups were further divided into subgroups (8th hour, 4th day, 7th day, 14th day, and 21st day). Using enzyme-linked immunosorbent assay, the serum level of platelet-derived growth factor-BB was determined. Friedman's test or the analysis of variance test was applied, and p-values under 0.05 were regarded as significant. The PRP+SVFs group had the highest mean PDGF-BB levels overall on days 4, 7, 14 and 21 (1.81±1.04 pg/ml, 1.24±0.41 pg/ml, 2.21±0.60 pg/ml, and 3.10±1.02 pg/ml, respectively). The repeated ANOVA test (Mauchly's test of sphericity) resulted in a p-value of 0.556 (>0.05), indicating that the variance of the PDGF-BB data was the same. Additionally, a p-value of 0.001 demonstrated a significant difference in the levels of PDGF-BB between the groups. Overall, the mean PDGF-BB level by day differed significantly between the PRP+SVFs group and the Vaseline group, the negative control group, and the group of healthy rats.
实验研究表明，基质血管成分和富血小板血浆对深度真皮烧伤愈合的效果令人鼓舞。在本研究中，我们通过测量血小板衍生生长因子（PDGF）-BB的血液水平，评估了它们对全层烧伤的影响。48只Wistar大鼠被分为四个主要组：A组大鼠接受SVF和PRP联合注射，B组大鼠局部涂抹凡士林，C组大鼠接受安慰剂注射，D组大鼠未烧伤。根据安乐死的日期，这些组进一步分为亚组（第8小时、第4天、第7天、第14天和第21天）。使用酶联免疫吸附测定法测定血清中血小板衍生生长因子-BB的水平。应用Friedman检验或方差分析检验，p值小于0.05被视为具有显著性。在第4天、第7天、第14天和第21天，PRP+SVFs组的平均PDGF-BB水平总体上最高（分别为1.81±1.04 pg/ml、1.24±0.41 pg/ml、2.21±0.60 pg/ml和3.10±1.02 pg/ml）。重复方差分析检验（Mauchly球形检验）得出的p值为0.556（>0.05），表明PDGF-BB数据的方差相同。此外，p值为0.001表明各组之间PDGF-BB水平存在显著差异。总体而言，PRP+SVFs组与凡士林组、阴性对照组和健康大鼠组之间，按天计算的平均PDGF-BB水平存在显著差异。
