Florio Daniela, Gridelli Stefano, Fioravanti Maria Letizia, Zanoni Renato Giulio
J Zoo Wildl Med. 2016 Mar;47(1):351-3. doi: 10.1638/2015-0064.1.
This report describes a case of the first isolation of Tenacibaculum maritimum from a captive-bred adult female sand tiger shark (Carcharias taurus) housed at the Cattolica Aquarium (Italy). The animal showed, between the second dorsal fin and the precaudal pit, skin lesions characterized by the presence of abundant whitish necrotic tissue. Through routine bacteriological examination, a bacterium was isolated from a skin lesion and subsequently identified as T. maritimum by phenotypic characters and species-specific polymerase chain reaction. The antimicrobial sensitivity of the isolated strain was evaluated for 11 antimicrobial agents by disk diffusion method. Antibiotic therapy was conducted with enrofloxacin at 10 mg kg(-1) i.m. on alternate days for 10 days. One month after the end of treatment skin lesions showed complete resolution and the shark recovered completely. The case presented here represents the first report of infection by T. maritimum in a sand tiger shark and highlights the potential pathogenic role of this microorganism in elasmobranchs kept in an aquarium.
本报告描述了从意大利卡托利卡水族馆饲养的一条人工养殖成年雌性沙虎鲨（噬人鲨）首次分离出海生屈挠杆菌的病例。该动物在第二背鳍和尾前凹之间出现皮肤病变，其特征为存在大量白色坏死组织。通过常规细菌学检查，从皮肤病变处分离出一种细菌，随后通过表型特征和种特异性聚合酶链反应鉴定为海生屈挠杆菌。采用纸片扩散法对分离菌株进行了11种抗菌剂的药敏试验。采用恩诺沙星10mg/kg肌肉注射，隔日一次共10天进行抗生素治疗。治疗结束后一个月，皮肤病变完全消退，鲨鱼完全康复。此处报告的病例是沙虎鲨感染海生屈挠杆菌的首例报告，突出了这种微生物在饲养于水族馆的板鳃亚纲动物中的潜在致病作用。
