Ren Yuxi, Bi Haining, Zhang Ji, Yin Qi, Zhang Xue, Gong Xuemei, Li Yaojiao, Shi Jifang
Department of Gynecology and Obstetrics, The First Affiliated Hospital of Dali University, Dali, 671000, People's Republic of China.
School of AI and Advanced Computing, Xi'an Jiaotong-Liverpool University, Suzhou, 215400, People's Republic of China.
Int J Womens Health. 2025 May 21;17:1473-1484. doi: 10.2147/IJWH.S504035. eCollection 2025.
Preeclampsia (PE) is a serious pregnancy-specific disorder that poses significant risks to maternal and fetal health, with severe preeclampsia (SPE) being a particularly life-threatening complication. The objective of this study is to investigate the effects and clinical significance of irisin in pregnant women with severe preeclampsia (SPE). Irisin levels in the serum and placental tissues of healthy pregnant women and those with early- and late-onset SPE were measured and compared.
A total of 70 pregnant women treated at our hospital from January to November 2023 were selected for this study. The participants were divided into three groups: 20 women with early-onset severe preeclampsia (ES-PE group), 20 women with late-onset severe preeclampsia (LS-PE group), and 30 healthy pregnant women (control group). Fasting peripheral blood samples (5 mL) were collected from each participant, and placental tissues were obtained after delivery. Irisin levels in serum were measured using enzyme-linked immunosorbent assays (ELISA) with a commercial kit, and irisin expression in placental tissues was assessed by immunohistochemistry (IHC) with a rabbit anti-irisin antibody. The modes of delivery were also recorded.
The concentrations of irisin in both serum and placental tissues were significantly higher among pregnant women in the control group compared to the ES-PE and LS-PE groups. There was a significant difference between the control group and the ES-PE and LS-PE groups in the mode of delivery. Additionally, a significant positive correlation was identified between the serum irisin concentration and its differential expression in placental tissues, while there was a significant negative correlation between irisin levels in both serum and placental tissue and systolic and/or diastolic blood pressure.
Reduced serum and placental irisin levels in pregnant women with SPE were associated with the onset and progression of SPE and may serve as a potential biological marker for SPE screening.
子痫前期（PE）是一种严重的妊娠特异性疾病，对母婴健康构成重大风险，重度子痫前期（SPE）是一种特别危及生命的并发症。本研究的目的是探讨鸢尾素在重度子痫前期（SPE）孕妇中的作用及临床意义。测量并比较健康孕妇以及早发型和晚发型SPE孕妇血清和胎盘组织中的鸢尾素水平。
本研究选取了2023年1月至11月在我院接受治疗的70名孕妇。参与者分为三组：20例早发型重度子痫前期妇女（ES-PE组）、20例晚发型重度子痫前期妇女（LS-PE组）和30例健康孕妇（对照组）。从每位参与者采集5 mL空腹外周血样本，并在分娩后获取胎盘组织。使用商业试剂盒通过酶联免疫吸附测定（ELISA）测量血清中的鸢尾素水平，并用兔抗鸢尾素抗体通过免疫组织化学（IHC）评估胎盘组织中鸢尾素的表达。还记录了分娩方式。
与ES-PE组和LS-PE组相比，对照组孕妇血清和胎盘组织中鸢尾素的浓度显著更高。对照组与ES-PE组和LS-PE组在分娩方式上存在显著差异。此外，血清鸢尾素浓度与其在胎盘组织中的差异表达之间存在显著正相关，而血清和胎盘组织中的鸢尾素水平与收缩压和/或舒张压之间存在显著负相关。
SPE孕妇血清和胎盘鸢尾素水平降低与SPE的发生和进展相关，可能作为SPE筛查的潜在生物学标志物。
