Vladitsi Magdalini, Kalogiouri Natasa P, Mourouzis Petros, Kabir Abuzar, Furton Kenneth, Samanidou Victoria F
School of Chemistry, Laboratory of Analytical Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
School of Dental Tissues Pathology and Therapeutics, Division of Operative Dentistry, Faculty of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Sep Sci. 2025 May;48(5):e70183. doi: 10.1002/jssc.70183.
A novel, simple capsule phase microextraction combined with high-pressure liquid chromatography coupled with ultraviolet detection analytical method was developed for the simultaneous determination of organic monomers that can be released from dental resins [bisphenol-A (BPA), bisphenol A glycerolatedimethacrylate (Bis-GMA), triethylene glycol dimethacrylate (TEGDMA), and urethane dimethacrylate (UDMA)] in cola drinks. The critical parameters that affect the extraction, such as the selection of the capsule type and size, extraction time, type, and volume of elution solvent, stirring rate during the extraction, and desorption time, were optimized. The method was subsequently validated in terms of selectivity, linearity, accuracy, precision, and robustness and showed satisfactory results. The limits of detection and quantitation were equal to 0.06 and 0.2 ng/µL, respectively, and the recoveries ranged between 93.3% and 106.9%. The method was employed in the determination of monomers released from cola drinks. For this purpose, cola refreshments were incubated with dental resins for 24 h and 7 days, and the proposed method was successfully used for monitoring the release in cola samples.
开发了一种新颖、简单的胶囊相微萃取结合高压液相色谱-紫外检测分析方法，用于同时测定可乐饮料中可从牙科树脂释放的有机单体[双酚A（BPA）、双酚A甘油化二甲基丙烯酸酯（Bis-GMA）、三乙二醇二甲基丙烯酸酯（TEGDMA）和聚氨酯二甲基丙烯酸酯（UDMA）]。对影响萃取的关键参数进行了优化，如胶囊类型和尺寸的选择、萃取时间、洗脱溶剂的类型和体积、萃取过程中的搅拌速率以及解吸时间。随后，该方法在选择性、线性、准确度、精密度和稳健性方面得到验证，并显示出令人满意的结果。检测限和定量限分别为0.06和0.2 ng/µL，回收率在93.3%至106.9%之间。该方法用于测定可乐饮料中释放的单体。为此，将可乐饮料与牙科树脂孵育24小时和7天，所提出的方法成功用于监测可乐样品中的释放情况。
