Department of Science & Technology, Department of Urology, Nano Medical Innovation & Collaboration Group (NMICG), The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou, China.
Department of Respiratory and Critical Care Medicine, Nano Medical Innovation & Collaboration Group (NMICG), The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou, China.
Environ Res. 2023 Oct 15;235:116700. doi: 10.1016/j.envres.2023.116700. Epub 2023 Jul 20.
A novel biodegradable dextrin-based nanocomposite, involving polypyrrole (PPy) and hydrophilic dextrin (Dex) (PPy@Dex) was prepared using in-situ radical chemical polymerization technique. The obtained PPy@Dex bionanocomposite was fully characterized by FT-IR, XRD, FESEM, and DSC methods. The exceptional properties such as biocompatibility, high surface area, the proper functional group on the surface, and outstanding electrical conductivity of synthesized bionanocomposite made it a superior candidate over biomolecules immobilization. Electrochemical observations revealed that the PPy@Dex-coated glassy carbon electrode (GCE) demonstrated improved performance, making it a suitable substrate for immobilizing hemoglobin (Hb) and constructing an efficient biosensor. The resulting biosensor, named Hb-PPy@Dex/GCE, exhibited high activity in the reduction of hydrogen peroxide (HO). Amperometric examinations demonstrated an extensive linear range from 2 to 350 μM for Hb-PPy@Dex/GCE. The detection limit of the proposed approach was calculated to be 0.54 μM, following the S/N = 3 protocol.
一种新型可生物降解的基于糊精的纳米复合材料，涉及聚吡咯（PPy）和亲水性糊精（Dex）（PPy@Dex），使用原位自由基化学聚合技术制备。通过 FT-IR、XRD、FESEM 和 DSC 方法对获得的 PPy@Dex 仿生纳米复合材料进行了充分的表征。合成的仿生纳米复合材料具有生物相容性、高比表面积、表面适当的官能团和出色的导电性等优异性能，使其成为生物分子固定化的首选。电化学观察表明，PPy@Dex 涂覆的玻碳电极（GCE）表现出改善的性能，使其成为固定血红蛋白（Hb）和构建高效生物传感器的合适基底。所得生物传感器命名为 Hb-PPy@Dex/GCE，在还原过氧化氢（HO）方面表现出高活性。安培检测表明，Hb-PPy@Dex/GCE 的线性范围从 2 到 350 μM 很宽。根据 S/N=3 协议，该方法的检测限计算为 0.54 μM。
