Xie Agui, Zhang Xingjiang, Huang Qing, Wu Jianxin
Skin Health and Cosmetic Development & Evaluation Laboratory, China Pharmaceutical University, Nanjing 211198, China.
Metabolites. 2025 Apr 26;15(5):292. doi: 10.3390/metabo15050292.
Sensitive skin exhibits impaired skin barrier function. The lipid composition of the skin, a pivotal element within the stratum corneum's "brick-and-mortar" structure, plays a dual role: it is integral to cell differentiation processes and serves as a vital nutrient reservoir for cutaneous microbiota, thereby influencing the skin's microecological balance. There is a notable research gap concerning the comparative analysis of physiological parameters and lipid profiles among individuals with sensitive dry skin (SDS), sensitive oily skin (SOS), and healthy skin (HS). A total of 95 females (18-25 years) were grouped: SDS ( = 32), SOS ( = 31), and HS ( = 32). Stratum corneum water content, oil content, and TEWL were measured. Lipids from sebaceous glands and stratum corneum (tape-stripping) underwent UPLC-QTOF-MS analysis. Differential lipids were identified via OPLS-DA, volcano plots, and LMSD. In terms of physiological indicators, notable disparities emerged in oil content and stratum corneum water content between the SOS and both the HS and the SDS. Sensitive skin, whether dry or oily, displayed a higher transepidermal water loss (TEWL) value than healthy skin, reflecting a declined state of skin barrier function. Regarding the sebum samples, the relative percentages of sphingolipids (SP) and glycerophospholipids (GP) were significantly higher in SDS. Regarding the stratum corneum samples, the percentages of SP in SDS were significantly higher. This study, for the first time, conducted a comprehensive analysis of the skin's physiological properties, lipidomics of sebum, and stratum corneum lipids among groups with SDS, SOS, and HS. These observations indicate a profound association between skin barrier dysfunction in SDS individuals and, in particular, sphingolipids (SP).
敏感性皮肤表现出皮肤屏障功能受损。皮肤的脂质组成是角质层“砖-灰浆”结构中的关键要素，具有双重作用：它是细胞分化过程所必需的，并且是皮肤微生物群的重要营养储备库，从而影响皮肤的微生态平衡。在敏感性干性皮肤（SDS）、敏感性油性皮肤（SOS）和健康皮肤（HS）个体之间，关于生理参数和脂质谱的比较分析存在显著的研究空白。总共95名女性（18至25岁）被分组：SDS组（n = 32）、SOS组（n = 31）和HS组（n = 32）。测量了角质层含水量、油脂含量和经表皮水分流失（TEWL）。对皮脂腺和角质层的脂质（胶带剥离法获取）进行超高效液相色谱-四极杆飞行时间质谱（UPLC-QTOF-MS）分析。通过正交偏最小二乘法判别分析（OPLS-DA）、火山图和脂质多变量统计差异分析（LMSD）鉴定差异脂质。在生理指标方面，SOS组与HS组和SDS组之间在油脂含量和角质层含水量上出现了显著差异。敏感性皮肤，无论是干性还是油性，其经表皮水分流失（TEWL）值均高于健康皮肤，这反映出皮肤屏障功能的下降状态。在皮脂样本方面，鞘脂（SP）和甘油磷脂（GP）的相对百分比在SDS组中显著更高。在角质层样本方面，SDS组中SP的百分比显著更高。本研究首次对SDS组、SOS组和HS组的皮肤生理特性、皮脂脂质组学和角质层脂质进行了全面分析。这些观察结果表明，SDS个体的皮肤屏障功能障碍与特别是鞘脂（SP）之间存在密切关联。
