Chen Tian, Wang Juan, Mao Zhenxing
Division of Public Health Service and Safety Assessment, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China.
NMPA Key Laboratory for Monitoring and Evaluation of Cosmetics, Shanghai 200336, China.
Metabolites. 2023 Apr 13;13(4):553. doi: 10.3390/metabo13040553.
This cross-sectional study aimed to investigate differences in skin surface lipids (SSL) and explore related metabolic pathways among females of different ages in Henan Province. Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was used to determine the lipid composition of the skin surface of 58 female volunteers who were divided into three age groups. Statistical analysis was performed using Progenesis QI, Ezinfo, and MetaboAnalyst. Multivariate and enrichment analysis were used to identify the different SSL among the groups. A total of 530 lipid entities were identified and classified into eight classes. Among these, 63 lipids were significantly different between the groups. Lower levels of glycerolipids (GLs) and sphingolipids (SPs) were observed in the middle-aged group, while higher levels of GLs were found in the elder group. GLs belonged to the largest and statistically significant enrichment of lipid metabolic pathways, and the lipid individuals enriched to the sphingoid bases metabolism were the most and statistically significant. These findings suggest that there are differences in hand SSL among females of different ages, which may be related to GLs and sphingoid bases metabolism.
这项横断面研究旨在调查河南省不同年龄段女性的皮肤表面脂质（SSL）差异，并探索相关代谢途径。采用超高效液相色谱四极杆飞行时间质谱（UPLC-QTOF-MS）测定了58名女性志愿者皮肤表面的脂质组成，这些志愿者被分为三个年龄组。使用Progenesis QI、Ezinfo和MetaboAnalyst进行统计分析。采用多变量和富集分析来识别各组之间不同的SSL。共鉴定出530种脂质实体，并分为八类。其中，63种脂质在各组之间存在显著差异。中年组甘油olipids（GLs）和鞘脂（SPs）水平较低，而老年组GLs水平较高。GLs属于脂质代谢途径中最大且具有统计学意义的富集类别，富集到鞘氨醇碱基代谢的脂质个体数量最多且具有统计学意义。这些发现表明，不同年龄段女性手部SSL存在差异，这可能与GLs和鞘氨醇碱基代谢有关。
