Department of Cosmetics, School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, China.
Int J Mol Sci. 2024 Oct 14;25(20):11033. doi: 10.3390/ijms252011033.
Skin barrier impairment is becoming increasingly common due to changes in lifestyle and modern living environments. Oily sensitive skin (OSS) is a condition that is characterized by an impaired skin barrier. Thus, examining the differences between OSS and healthy skin will enable a more objective evaluation of the characteristics of OSS and facilitate investigations of potential treatments. Initially, a self-assessment questionnaire was used to identify patients with OSS. Biophysical measurements and LAST scores were used to determine whether skin barrier function was impaired. Epidermal biophysical properties, including skin hydration, transepidermal water loss (TEWL), sebum content, erythema index (EI), and a* value, were measured with noninvasive instruments. We subsequently devised a noninvasive D-square sampling technique to identify changes in the skin metabolome in conjunction with an untargeted metabolomics analysis with an Orbitrap Q ExactiveTM series mass spectrometer. In the stratum corneum of 47 subjects, 516 skin metabolites were identified. In subjects with OSS, there was an increase in the abundance of 15 metabolites and a decrease in the abundance of 48 metabolites. The participants with OSS were found to have the greatest disruptions in sphingolipid and amino acid metabolism. The results revealed that an impaired skin barrier is present in patients with OSS and offers a molecular target for screening for skin barrier damage.
由于生活方式和现代生活环境的变化，皮肤屏障损伤变得越来越普遍。油性敏感皮肤（OSS）是一种以皮肤屏障受损为特征的病症。因此，研究 OSS 和健康皮肤之间的差异将能够更客观地评估 OSS 的特征，并有助于研究潜在的治疗方法。首先，使用自我评估问卷来识别 OSS 患者。使用皮肤生物物理测量和 LAST 评分来确定皮肤屏障功能是否受损。使用非侵入性仪器测量表皮生物物理特性，包括皮肤水分、经表皮水分流失（TEWL）、皮脂含量、红斑指数（EI）和 a* 值。随后，我们设计了一种非侵入性的 D-square 采样技术，结合使用 Orbitrap Q ExactiveTM 系列质谱仪的非靶向代谢组学分析来识别皮肤代谢组的变化。在 47 名受试者的角质层中，鉴定出 516 种皮肤代谢物。在 OSS 受试者中，有 15 种代谢物的丰度增加，48 种代谢物的丰度减少。OSS 患者的神经酰胺和氨基酸代谢受到的干扰最大。研究结果表明，OSS 患者的皮肤屏障受损，为筛选皮肤屏障损伤提供了一个分子靶点。
