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当前抗地高辛抗体缺乏特异性，以及制备一种能识别地高辛碳水化合物部分的新型特异性多克隆抗体。

Lack of specificity of current anti-digoxin antibodies, and preparation of a new, specific polyclonal antibody that recognizes the carbohydrate moiety of digoxin.

作者信息

Thong B, Soldin S J, Lingwood C A

出版信息

Clin Chem. 1985 Oct;31(10):1625-31.

PMID:4042328
Abstract

Current immunoassays for digoxin do not distinguish digoxin from its glycosidic metabolites. We have synthesized a novel digoxin/bovine serum albumin conjugate via reductive ozonolysis of the lactone ring such that the carbohydrate moiety of digoxin remains intact. Antibodies raised against this conjugate show minimal cross reactivity to digoxigenin, bisdigitoxide, monodigitoxide, digoxigenin, and digitoxin. With this antibody, digoxin can be measured in the presence of these metabolites.

摘要

目前用于地高辛的免疫测定法无法区分地高辛与其糖苷代谢物。我们通过内酯环的还原性臭氧分解合成了一种新型地高辛/牛血清白蛋白缀合物，使得地高辛的碳水化合物部分保持完整。针对这种缀合物产生的抗体与洋地黄毒苷元、双去氧地高辛、单去氧地高辛、洋地黄毒苷元和洋地黄毒苷的交叉反应最小。使用这种抗体，可以在这些代谢物存在的情况下测定地高辛。

相似文献

1
Lack of specificity of current anti-digoxin antibodies, and preparation of a new, specific polyclonal antibody that recognizes the carbohydrate moiety of digoxin.当前抗地高辛抗体缺乏特异性，以及制备一种能识别地高辛碳水化合物部分的新型特异性多克隆抗体。
Clin Chem. 1985 Oct;31(10):1625-31.
2
Cross-reactivity of anti-digoxin antibodies with digitoxin depends on tracer structure.抗地高辛抗体与洋地黄毒苷的交叉反应性取决于示踪剂结构。
Clin Chem. 1992 Nov;38(11):2228-32.
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[Production and characterization of a high affinity monoclonal antibody with digoxin and digitoxin specificity].
Allerg Immunol (Leipz). 1990;36(4):343-50.
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[Affinity of polar digoxin and digitoxin metabolites for digoxin and digitoxin antibodies].[极性地高辛和洋地黄毒苷代谢物与地高辛和洋地黄毒苷抗体的亲和力]
Arzneimittelforschung. 1977;27(3):649-53.
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Bidirectional (positive/negative) interference in a digoxin immunoassay: importance of antibody specificity.地高辛免疫测定中的双向（正向/负向）干扰：抗体特异性的重要性。
Ther Drug Monit. 1998 Jun;20(3):352-7. doi: 10.1097/00007691-199806000-00019.
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Production and characterization of monoclonal and polyclonal antibodies against digoxin.抗地高辛单克隆抗体和多克隆抗体的制备与特性鉴定
Eisei Shikenjo Hokoku. 1990(108):29-33.
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Production and properties of digoxin-specific antibodies.地高辛特异性抗体的制备及其性质
Ann N Y Acad Sci. 1974;242(0):717-30. doi: 10.1111/j.1749-6632.1974.tb19133.x.
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Preparation and antigenic properties of digitoxin-bovine serum albumin conjugates linked at the digitoxose C-3' and C-3" positions.
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Preparation of specific antisera to digoxin by using digoxin C-3' and C-3" hemisuccinate-bovine serum albumin conjugates.使用地高辛C-3'和C-3''半琥珀酸酯-牛血清白蛋白偶联物制备地高辛特异性抗血清。
Biol Pharm Bull. 2000 Aug;23(8):906-10. doi: 10.1248/bpb.23.906.
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The effectiveness of digoxin-specific F(ab')2-antibody fragments in the treatment of digitoxin poisoning: experimental investigations in the cat.地高辛特异性F(ab')2抗体片段治疗洋地黄毒苷中毒的有效性：猫的实验研究
Eur J Clin Invest. 1980 Apr;10(2 Pt 1):93-7. doi: 10.1111/j.1365-2362.1980.tb02066.x.

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