Erkell L J, Ryd W, Hagmar B
Clin Exp Metastasis. 1985 Jan-Mar;3(1):1-5. doi: 10.1007/BF01758949.
Cell debris is inevitably produced by the mechanical and enzymatic procedures used to dissociate solid tumors. We investigated the influence of such debris on the i.v. and s.c. transplantability of B16 melanoma cells. Cell suspensions were centrifuged on a Percoll cushion to remove the debris, and the purified preparations were compared to untreated control suspensions. The i.v. injection of a massive dose (10(6) cells) of unpurified cells killed the animals within a few minutes, while the same amount of purified cells left the animals unaffected. When 5 X 10(5) cells were given, animals receiving purified cell suspensions had more extrapulmonary tumors at their time of death than those given unpurified cells. However, with a lower cell dose, 2 X 10(4) cells, there were no such differences in tumor incidence or distribution.
在解离实体瘤所采用的机械和酶促操作过程中，不可避免地会产生细胞碎片。我们研究了此类碎片对B16黑色素瘤细胞静脉注射和皮下移植能力的影响。细胞悬液在Percoll垫层上进行离心以去除碎片，将纯化后的制剂与未处理的对照悬液进行比较。静脉注射大量（10⁶个细胞）未纯化的细胞会在几分钟内导致动物死亡，而相同数量的纯化细胞则不会对动物产生影响。当注射5×10⁵个细胞时，接受纯化细胞悬液的动物在死亡时肺外肿瘤比接受未纯化细胞的动物更多。然而，当细胞剂量较低，为2×10⁴个细胞时，肿瘤发生率或分布没有此类差异。
