Kim Dae-Youn, Kim Tae-Jong
Department of Forest Products and Biotechnology, Kookmin University, Seoul 02707, Republic of Korea.
Antibiotics (Basel). 2025 Apr 22;14(5):424. doi: 10.3390/antibiotics14050424.
: The persistence of poses a significant challenge in clinical treatments because of its ability to withstand antibiotic therapy. This study assessed the role of quercetin in promoting bacterial dormancy and persistence through ATP depletion and revealed its potential impact on antibiotic tolerance. : To assess the effects of quercetin on bacterial metabolism and persistence, cultures were treated with quercetin, and intracellular ATP levels were then measured. The effect of quercetin on persister cell formation was assessed using antibiotic exposure assays, including pre-treatment and post-treatment strategies. : Quercetin treatment significantly depleted intracellular ATP levels in a dose-dependent manner, suggesting the presence of metabolic stress. This ATP depletion correlated with increased persister cell formation across multiple antibiotic treatments, indicating that quercetin-induced dormancy enhances bacterial persistence. Notably, quercetin pre-treatment further increased persister cell counts, while delayed quercetin administration increased persister cell survival, highlighting the influence of the timing of metabolic stress on persistence outcomes. : Quercetin promotes bacterial persistence by inducing ATP depletion and metabolic dormancy. Although quercetin's bactericidal properties may initially impair bacterial growth, its potential to enhance persistence underscores the complexity of its effects. Further research is necessary to determine optimal strategies for harnessing the antimicrobial properties of quercetin while minimizing its persistence-promoting effects.
由于其具有耐受抗生素治疗的能力，[细菌的某种特性]的持续存在对临床治疗构成了重大挑战。本研究评估了槲皮素通过消耗ATP促进细菌休眠和持续存在的作用，并揭示了其对抗生素耐受性的潜在影响。：为了评估槲皮素对细菌代谢和持续存在的影响，用槲皮素处理[细菌]培养物，然后测量细胞内ATP水平。使用抗生素暴露试验评估槲皮素对持留菌形成的影响，包括预处理和后处理策略。：槲皮素处理以剂量依赖性方式显著降低细胞内ATP水平，表明存在代谢应激。这种ATP消耗与多种抗生素治疗中持留菌形成的增加相关，表明槲皮素诱导的休眠增强了细菌的持续存在。值得注意的是，槲皮素预处理进一步增加了持留菌数量，而延迟给予槲皮素则增加了持留菌的存活率，突出了代谢应激时机对持续存在结果的影响。：槲皮素通过诱导ATP消耗和代谢休眠来促进细菌的持续存在。尽管槲皮素的杀菌特性最初可能会损害细菌生长，但其增强持续存在的潜力强调了其作用的复杂性。有必要进行进一步研究，以确定在利用槲皮素抗菌特性的同时将其促进持续存在的影响降至最低的最佳策略。
