Ghanaati Shahram, Dohle Eva, Schick Fabian, Lechner Johann
FORM, Frankfurt Orofacial Regenerative Medicine, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Johann Wolfgang Goethe University, 60590 Frankfurt, Germany.
ABIS e.V. (Academy for Biological Innovations in Surgery Formally Known as SBCB e.V.), Society for Blood Concentrate and Biomaterials e.V., 60435 Frankfurt, Germany.
Biomedicines. 2025 May 8;13(5):1144. doi: 10.3390/biomedicines13051144.
Immune cells are integral to bone homeostasis, including the repair and remodeling of bone tissue. Chronic dysregulation within this osteoimmune network can lead to bone marrow defects of the jaw (BMDJ), particularly fatty degenerative osteonecrosis of the jaw (FDOJ). These localized pathologies are implicated in systemic immune dysfunctions. This study is designed to determine whether BMDJ/FDOJ samples are indicative of medullary bone pathology by evaluating FDOJ gene expression patterns using quantitative real-time PCR. Comparative analyses between pathological and healthy samples evaluated the dysregulation of key molecular pathways. BMDJ/FDOJ samples showed significant upregulation of inflammatory mediators, including CCL5/RANTES, VEGF, IGF and KOR, and downregulation of structural proteins, such as collagen types I, II and IV, and osteogenesis-associated factors, such as SP7. The study provides new insights into the molecular mechanisms of BMDJ/FDOJ by identifying potential molecular changes suggesting a pro-inflammatory state in the affected jawbone which may contribute to systemic immune dysregulation. The findings are consistent with morphologic observations of BMDJ/FDOJ in degenerated jawbone and underscore the need for integrative approaches in dentistry and medicine while highlighting BMDJ/FDOJ as a potential target for therapeutic and preventive strategies against systemic diseases and emphasizing its clinical significance.
免疫细胞对于骨稳态不可或缺，包括骨组织的修复和重塑。这种骨免疫网络内的慢性失调可导致颌骨骨髓缺陷（BMDJ），尤其是颌骨脂肪变性性骨坏死（FDOJ）。这些局部病变与全身免疫功能障碍有关。本研究旨在通过使用定量实时PCR评估FDOJ基因表达模式，确定BMDJ/FDOJ样本是否指示髓质骨病理。病理样本与健康样本之间的比较分析评估了关键分子途径的失调。BMDJ/FDOJ样本显示炎症介质显著上调，包括CCL5/RANTES、VEGF、IGF和KOR，以及结构蛋白如I型、II型和IV型胶原蛋白和成骨相关因子如SP7的下调。该研究通过识别潜在的分子变化，为BMDJ/FDOJ的分子机制提供了新的见解，这些变化表明受影响的颌骨处于促炎状态，这可能导致全身免疫失调。这些发现与退化颌骨中BMDJ/FDOJ的形态学观察结果一致，强调了牙科和医学中采用综合方法的必要性，同时突出了BMDJ/FDOJ作为针对全身性疾病的治疗和预防策略的潜在靶点，并强调了其临床意义。
