Wesdorp Marinus A, Schwab Andrea, Bektas Ezgi Irem, Narcisi Roberto, Eglin David, Stoddart Martin J, Van Osch Gerjo J V M, D'Este Matteo
AO Research Institute Davos, AO Foundation, Davos Platz, Switzerland.
Department of Orthopaedics and Sports Medicine, Erasmus MC, University Medical Center Rotterdam, the Netherlands.
Bioact Mater. 2022 Jul 2;20:627-637. doi: 10.1016/j.bioactmat.2022.05.036. eCollection 2023 Feb.
Neutrophils play a pivotal role in orchestrating the immune system response to biomaterials, the onset and resolution of chronic inflammation, and macrophage polarization. However, the neutrophil response to biomaterials and the consequent impact on tissue engineering approaches is still scarcely understood. Here, we report an culture model that comprehensively describes the most important neutrophil functions in the light of tissue repair. We isolated human primary neutrophils from peripheral blood and exposed them to a panel of hard, soft, naturally- and synthetically-derived materials. The overall trend showed increased neutrophil survival on naturally derived constructs, together with higher oxidative burst, decreased myeloperoxidase and neutrophil elastase and decreased cytokine secretion compared to neutrophils on synthetic materials. The culture model is a step to better understand the immune modulation elicited by biomaterials. Further studies are needed to correlate the neutrophil response to tissue healing and to elucidate the mechanism triggering the cell response and their consequences in determining inflammation onset and resolution.
中性粒细胞在协调免疫系统对生物材料的反应、慢性炎症的发生和消退以及巨噬细胞极化方面发挥着关键作用。然而，中性粒细胞对生物材料的反应以及随之对组织工程方法产生的影响仍鲜为人知。在此，我们报告一种培养模型，该模型根据组织修复全面描述了最重要的中性粒细胞功能。我们从外周血中分离出人类原代中性粒细胞，并将它们暴露于一系列硬的、软的、天然和合成来源的材料中。总体趋势表明，与合成材料上的中性粒细胞相比，天然来源构建体上的中性粒细胞存活率增加，同时氧化爆发增强、髓过氧化物酶和中性粒细胞弹性蛋白酶减少以及细胞因子分泌减少。该培养模型是更好地理解生物材料引发的免疫调节的重要一步。需要进一步的研究来关联中性粒细胞反应与组织愈合，并阐明触发细胞反应的机制及其在确定炎症发生和消退中的后果。
