Wei Jiao, Fu Yifan, Feng Shinan, Zhang Jinjing, Zhang Yuanyuan, Yu Jiaxing, Kang Pengtian, Wu Chenglong, Mi Haifeng
National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition (Zhejiang), Huzhou University, 759 East 2nd Road, Huzhou 313000, China.
Gansu Provincial Aquatic Technology Extension Station, 113 Zhongshan Road, Lanzhou 730030, China.
Animals (Basel). 2025 May 13;15(10):1404. doi: 10.3390/ani15101404.
This research investigated the growth, serum biochemistry, antioxidant capability, and immunity impact of black carp fed degossypolled cottonseed protein replacing fishmeal at the levels of 0%, 10%, 20%, 30%, 40%, and 50% (DCP0, DCP10, DCP20, DCP30, DCP40, and DCP50), respectively. The results showed there were no significant changes in growth among these test groups. The activities and mRNA expression levels of amylase and trypsin were heightened in conjunction with 30-40% DCP. Although the insulin contents were reduced with a rise in DCP content, 5-hydroxytryptamino was increased in the DCP40 and DCP50 groups. DCP40 could heighten the levels of low-density lipoprotein cholesterol, triglycerides, total cholesterol, and urea nitrogen. Although lower levels of DCP (≤20%) could increase the total antioxidant capacity compared with the DCP50 group, DCP50 could markedly heighten levels of catalase, glutathione S-transferase, HO, and malondialdehyde. Meanwhile, the mRNA levels of Mn-superoxide dismutase, glutathione reductase, glutathione peroxidase, glutamate-cysteine ligase regulatory subunit, and nuclear factor E2-related factor 2 were heightened in the DCP30 group compared with the DCP50 group. The levels of alkaline phosphatase, immunoglobulin M, and liver-expressed antimicrobial peptide 2 were markedly heightened in the liver of the DCP20 group compared with the DCP50 group. In conclusion, a suitable level of DCP (20%) could improve serum biochemical indices and hormone variation, enhance antioxidant capability, and increase immunity in black carp.
本研究调查了用脱毒棉籽蛋白替代鱼粉水平分别为0%、10%、20%、30%、40%和50%（DCP0、DCP10、DCP20、DCP30、DCP40和DCP50）的饲料喂养的青鱼的生长、血清生化指标、抗氧化能力及免疫影响。结果表明，这些试验组之间的生长没有显著变化。淀粉酶和胰蛋白酶的活性及mRNA表达水平随着30%-40%的脱毒棉籽蛋白添加量而升高。虽然胰岛素含量随着脱毒棉籽蛋白含量的增加而降低，但在DCP40和DCP50组中5-羟色胺增加。DCP40会提高低密度脂蛋白胆固醇、甘油三酯、总胆固醇和尿素氮的水平。虽然较低水平的脱毒棉籽蛋白（≤20%）与DCP50组相比可提高总抗氧化能力，但DCP50可显著提高过氧化氢酶、谷胱甘肽S-转移酶、血红素加氧酶和丙二醛的水平。同时，与DCP50组相比，DCP30组中锰超氧化物歧化酶、谷胱甘肽还原酶、谷胱甘肽过氧化物酶、谷氨酸-半胱氨酸连接酶调节亚基和核因子E2相关因子2的mRNA水平升高。与DCP50组相比，DCP20组肝脏中碱性磷酸酶、免疫球蛋白M和肝脏表达的抗菌肽2的水平显著升高。总之，适宜水平的脱毒棉籽蛋白（20%）可改善青鱼的血清生化指标和激素变化，增强抗氧化能力，并提高免疫力。
