Roșca Oana-Janina, Coneac Georgeta-Hermina, Racoviceanu Roxana, Nistor Alexandru, Olariu Ioana-Viorica, Cotan Ana-Maria, Negrea-Ghiulai Roxana, Dehelean Cristina Adriana, Vlaia Lavinia Lia, Șoica Codruța Marinela
Department of Pharmacology-Pharmacotherapy, Faculty of Pharmacy, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania.
Discipline of Clinical Practical Skills, Department I Nursing, Faculty of Medicine, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania.
Pharmaceutics. 2025 Jun 29;17(7):853. doi: 10.3390/pharmaceutics17070853.
In burn injuries, wound healing effectiveness is complex and influenced significantly by the local biochemical environment and the physicochemical properties of topical preparations. pH lesions modulation can influence protection barrier integrity, inflammatory responses, and microbial colonization. Their antioxidant, antimicrobial, and anti-inflammatory properties, of the topical formulations enriched with plant extracts have demonstrated promising results. The aim of the study was to develop and characterize topical oleogel and hydrogel formulations containing ethanolic and hydroalcoholic extracts of medicinal plants (, , , and ), and to evaluate the impact of their physicochemical properties, rheological behavior, in contrast with the wound pH modulation, and healing efficacy in an experimental burn model. Second-degree burns were induced uniformly on Wistar rats using the validated RAPID-3D device. All formulations were applied daily for 21 days, and wound healing was assessed through several measurements specific to the wound surface, skin temperature, pH, and, last but not least, histological analyses. Formulations' physicochemical and rheological properties, including pH, viscosity, and spreadability, were also analyzed and systematically characterized. Oleogel formulations demonstrated superior wound healing performance compared to hydrogels. Formulations containing and extracts significantly reduced wound size, inflammation, and melanin production by days 9 and 21 ( < 0.05). The beneficial outcomes correlated strongly with formulation acidity (pH < 6), high viscosity, and enhanced thixotropic behavior, indicating improved adherence and sustained bioactive compound release. Histological evaluations confirmed enhanced epithelialization and reduced inflammation. Particularly and in oleogel formulations in ethanolic solvent effectively modulated wound pH, enhanced topical adherence, and improved burn wound healing. These findings highlight their potential clinical application and justify further clinical investigations.
在烧伤中，伤口愈合效果复杂，受到局部生化环境和局部制剂物理化学性质的显著影响。pH值对损伤的调节可影响保护屏障的完整性、炎症反应和微生物定植。富含植物提取物的局部制剂的抗氧化、抗菌和抗炎特性已显示出有前景的结果。本研究的目的是开发并表征含有药用植物（、、和）乙醇提取物和水醇提取物的局部油凝胶和水凝胶制剂，并评估其物理化学性质、流变行为（与伤口pH调节相比）以及在实验性烧伤模型中的愈合效果。使用经过验证的RAPID-3D装置在Wistar大鼠身上均匀诱导二度烧伤。所有制剂每天应用21天，并通过针对伤口表面、皮肤温度、pH值以及最后但同样重要的组织学分析等多项测量来评估伤口愈合情况。还对制剂的物理化学和流变性质，包括pH值、粘度和铺展性进行了分析和系统表征。与水凝胶相比，油凝胶制剂显示出卓越的伤口愈合性能。含有和提取物的制剂在第9天和第21天时显著减小了伤口大小、炎症和黑色素生成（<0.05）。这些有益结果与制剂酸度（pH<6）、高粘度和增强的触变性密切相关，表明粘附性改善且生物活性化合物释放持续。组织学评估证实上皮化增强且炎症减轻。特别是乙醇溶剂中的油凝胶制剂中的和有效地调节了伤口pH值，增强了局部粘附性，并改善了烧伤伤口愈合。这些发现突出了它们潜在的临床应用价值，并为进一步的临床研究提供了依据。
