Lee Dongho, Kim Min Jung, Cho Chang-Soo, Yang Ye Jin, Kim Jin-Kyung, Jeon Ryounghoon, An Sang-Hyun, Park Kwang Il, Cho Kwangrae
Department of Anesthesiology and Pain Medicine, College of Medicine, Inje University, Gimhae 47392, Republic of Korea.
College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea.
Antioxidants (Basel). 2025 May 1;14(5):548. doi: 10.3390/antiox14050548.
(1) Background: Rheumatoid arthritis (RA) is a chronic inflammatory condition known for its symptoms of joint damage and cartilage breakdown. Current treatments frequently result in adverse effects and show restricted efficacy in the long term. , a plant recognized for its bioactive properties, demonstrates promise in the treatment of inflammatory conditions. The objective of this study was to examine the therapeutic properties of water extract (DMWE) in RA through the utilization of in vitro and in vivo models. (2) Methods: Ultra-high-performance liquid chromatography (UPLC) analysis was used to identify bioactive compounds in DMWE. Antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical-scavenging assays. The in vitro experiments involved the treatment of CHON-001 cells with DMWE in order to assess its impacts on inflammation and matrix metalloproteinase (MMP) expression. The impact of DMWE on the Janus Kinase 2 (JAK2) and Signal Transducer and Activator of Transcription (STAT) signaling pathways was also assessed. RA was induced in Balb/c mice who were subsequently treated with varying doses of DMWE to assess its impact on joint morphology, edema, and body weight. (3) Results: DMWE demonstrated substantial antioxidant activity and hindered the expression of MMP-2 and MMP-8 in chondrocytes treated with IL-1β. It additionally inhibited the JAK2/STAT pathway and diminished inflammatory responses. Treatment with DMWE in living organisms led to a decrease in joint swelling, improved weight regains, and maintained joint structure, with higher doses exhibiting effects similar to those of the positive control, dexamethasone (Dexa). (4) Conclusions: DMWE was found to have excellent in vitro antioxidant and anti-inflammatory activities. In an RA-induced mouse model, DMWE-3 (500 mg/kg BW) was found to effectively treat RA by reducing the concentration of pro-inflammatory factors and preventing joint deformation.
(1) 背景：类风湿性关节炎（RA）是一种慢性炎症性疾病，以关节损伤和软骨破坏症状为特征。目前的治疗方法常常会产生不良反应，且长期疗效有限。[植物名称]是一种因其生物活性特性而闻名的植物，在炎症性疾病的治疗中显示出前景。本研究的目的是通过体外和体内模型来研究[植物名称]水提取物（DMWE）在类风湿性关节炎中的治疗特性。(2) 方法：采用超高效液相色谱（UPLC）分析来鉴定DMWE中的生物活性化合物。使用1,1-二苯基-2-苦基肼（DPPH）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）自由基清除试验来评估抗氧化活性。体外实验包括用DMWE处理CHON-001细胞，以评估其对炎症和基质金属蛋白酶（MMP）表达的影响。还评估了DMWE对Janus激酶2（JAK2）和信号转导及转录激活因子（STAT）信号通路的影响。在Balb/c小鼠中诱导类风湿性关节炎，随后用不同剂量的DMWE进行治疗，以评估其对关节形态、水肿和体重的影响。(3) 结果：DMWE表现出显著的抗氧化活性，并抑制了用白细胞介素-1β处理的软骨细胞中MMP-2和MMP-8的表达。它还抑制JAK2/STAT通路并减少炎症反应。在生物体中用DMWE治疗导致关节肿胀减轻、体重恢复改善以及关节结构得以维持，较高剂量表现出与阳性对照地塞米松（Dexa）相似的效果。(4) 结论：发现DMWE具有出色的体外抗氧化和抗炎活性。在类风湿性关节炎诱导的小鼠模型中，发现DMWE-3（500毫克/千克体重）通过降低促炎因子浓度和预防关节变形有效治疗类风湿性关节炎。
