Department of Microbiology, Pathology and Forensic Medicine, Faculty of Medicine, The Hashemite University, Zarqa, 13133, Jordan.
Faculty of Applied Medical Sciences, The Hashemite University, Zarqa, 13133, Jordan.
Int J Nanomedicine. 2024 Nov 23;19:12461-12481. doi: 10.2147/IJN.S479937. eCollection 2024.
Given the increasing frequency of drug-resistant bacteria and the limited progress in developing new antibiotics, it is necessary to explore new methods of combating microbial infections. Nanoparticles, particularly silver nanoparticles (Ag-NPs), have shown exceptional antibacterial characteristics; however, elevated concentrations of Ag-NPs can produce noticeable levels of toxicity in mammalian cells.
This study examined the potential synergistic effect of combining a low dosage of Ag-NPs and anti-pseudomonas drugs against (ATCC strain) and eleven clinical isolates from cystic fibrosis patients.
The Ag-NPs were chemically produced by utilizing a seed extract from and characterized via ultraviolet-visible spectroscopy and scanning electron microscopy. The broth microdilution technique was utilized to investigate the minimum inhibitory concentration (MIC) of Ag-NPs and eight antibiotics (Piperacillin, Ciprofloxacin, Levofloxacin, Meropenem, Amikacin, Ceftazidime, Gentamicin, Aztreonam). The fractional inhibitory concentration index (FICI) was determined via the checkerboard method to evaluate the synergistic effects of Ag-NPs and various antibiotics.
The biosynthesized Ag-NPs were uniformly spherical and measured around 15 nm in size. When combined with antibiotics, Ag-NP produced statistically significant reductions in the amount of antibiotics required to completely prevent growth for all strains. The findings revealed that the MIC of Ag-NPs was 15 ug/mL for all strains which decreased substantially when administered with antibiotics at a dose of 1.875-7.5 ug/mL. The majority of Ag-NP and antibiotic combinations exhibited a synergistic or partially synergistic impact. This was particularly noticeable in combinations containing Meropenem, Ciprofloxacin, and Aztreonam (in which the FIC index was less than or equal to 0.5).
The findings revealed that combining Ag-NPs with antibiotics was more effective than using Ag-NPs or antibiotics in isolation and that combinations of Ag-NPs and antimicrobial agents displayed synergistic activity against the majority of strains assessed.
鉴于耐药菌的频率不断增加，而开发新抗生素的进展有限，因此有必要探索新的方法来对抗微生物感染。纳米粒子，特别是银纳米粒子（Ag-NPs），具有出色的抗菌特性；然而，Ag-NPs 的高浓度会对哺乳动物细胞产生明显的毒性。
本研究探讨了将低剂量 Ag-NPs 与抗假单胞菌药物联合使用对（ATCC 株）和 11 株来自囊性纤维化患者的临床分离株的潜在协同作用。
Ag-NPs 通过利用 的种子提取物化学合成，并通过紫外-可见光谱和扫描电子显微镜进行表征。采用肉汤微量稀释技术研究 Ag-NPs 和八种抗生素（哌拉西林、环丙沙星、左氧氟沙星、美罗培南、阿米卡星、头孢他啶、庆大霉素、氨曲南）的最小抑菌浓度（MIC）。通过棋盘法测定部分抑菌浓度指数（FICI）来评估 Ag-NPs 与各种抗生素的协同作用。
生物合成的 Ag-NPs 呈均匀的球形，大小约为 15nm。当与抗生素联合使用时，Ag-NP 可显著降低所有菌株完全抑制生长所需的抗生素用量。结果表明，所有菌株的 Ag-NPs MIC 为 15μg/ml，当与抗生素联合使用时，剂量为 1.875-7.5μg/ml 时，Ag-NPs MIC 显著降低。Ag-NP 与大多数抗生素组合表现出协同或部分协同作用。这在含有美罗培南、环丙沙星和氨曲南的组合中尤为明显（其中 FIC 指数小于或等于 0.5）。
研究结果表明，与单独使用 Ag-NPs 或抗生素相比，将 Ag-NPs 与抗生素联合使用更有效，并且 Ag-NPs 与抗菌药物的组合对大多数评估的菌株表现出协同活性。
