Schiano Elisabetta, Novellino Ettore, Gámez Fernández Marta María, Tiekou Lorinczova Helena, Tenore Gian Carlo, Iannuzzo Fortuna, Patel Vinood B, Somavarapu Satyanarayana, Zariwala Mohammed Gulrez
Department of Pharmacy, University of Naples Federico II, via Domenico Montesano 59, 80131 Naples, Italy.
Healthcare Food Research Center, Inventia Biotech s.r.l., Strada Statale Sannitica KM 20.700, 81020 Caserta, Italy.
Antioxidants (Basel). 2023 Dec 31;13(1):63. doi: 10.3390/antiox13010063.
Pancreatic β-cells play a crucial role in maintaining glucose homeostasis, although they are susceptible to oxidative damage, which can ultimately impair their functionality. Thinned nectarines (TNs) have gained increasing interest due to their high polyphenol and abscisic acid (ABA) content, both of which possess antidiabetic properties. Nevertheless, the efficacy of these bioactive compounds may be compromised by limited stability and bioavailability in vivo. This study aimed to develop nanoformulations (NFs) containing pure ABA or a TN extract (TNE) at an equivalent ABA concentration. Subsequently, the insulinotropic and antioxidant potential of the NFs and their unformulated (free) forms were compared in MIN-6 pancreatic cells exposed to varying glucose (5.5 mM and 20 mM) and iron (100 µM) concentrations. NF-TNE treatment exhibited enhanced antioxidant activity compared to free TNE, while ABA-based groups showed no significant antioxidant activity. Moreover, MIN6 cells incubated with both high glucose and iron levels demonstrated significantly higher insulin AUC levels after treatment with all samples, with NF-TNE displaying the most pronounced effect. In conclusion, these results highlight the additional beneficial potential of TNE due to the synergistic combination of bioactive compounds and demonstrate the significant advantage of using a nanoformulation approach to further increase the benefits of this and similar phytobioactive molecules.
胰腺β细胞在维持葡萄糖稳态中起着关键作用，尽管它们易受氧化损伤，而这最终可能损害其功能。薄皮油桃（TNs）因其高多酚和脱落酸（ABA）含量而越来越受到关注，这两种物质都具有抗糖尿病特性。然而，这些生物活性化合物的功效可能会因体内稳定性和生物利用度有限而受到影响。本研究旨在开发含有等浓度纯ABA或TN提取物（TNE）的纳米制剂（NFs）。随后，在暴露于不同葡萄糖（5.5 mM和20 mM）和铁（100 µM）浓度的MIN-6胰腺细胞中，比较了NFs及其未配制（游离）形式的促胰岛素和抗氧化潜力。与游离TNE相比，NF-TNE处理表现出增强的抗氧化活性，而基于ABA的组未显示出显著的抗氧化活性。此外，在高葡萄糖和铁水平下培养的MIN6细胞在用所有样品处理后，胰岛素AUC水平显著更高，其中NF-TNE的效果最为明显。总之，这些结果突出了由于生物活性化合物的协同组合而使TNE具有的额外有益潜力，并证明了使用纳米制剂方法进一步提高这种及类似植物生物活性分子益处的显著优势。
