Zhu Angie, Reid Emmett, Jain Tilak, Mir Amatullah, Siddiqi Usmaan, Dunne Olivia, Hibino Narutoshi
Section of Cardiac Surgery, Department of Surgery, University of Chicago, 5841 S. Maryland Ave., Chicago, IL 60637, USA.
37degrees, 111 North Wabash Ave. Ste. 100, Chicago, IL 60602, USA.
Bioengineering (Basel). 2025 May 21;12(5):554. doi: 10.3390/bioengineering12050554.
Perfusion offers unique benefits to tissue-engineered systems, enhancing oxygen and nutrient transport, which improves tissue formation and growth. In this study, we present a novel and integrated portable perfusion system. Weighing < 10 lbs, the system can maintain continuous flow in a standard incubation environment (37 °C, 5% CO), effectively functioning as a portable perfusion and tissue culturing system. To characterize the perfusion system's flow parameters, we measured the volumetric flow rate across a range of pressures and found that the system could achieve flow velocities between 1.69 to 4.6 μm/s, which is similar to in vivo interstitial flow. Computational fluid dynamics revealed fully developed laminar flow within the sample-containing region of the perfusion system, helping ensure even fluid and nutrient distribution. To study the system's compatibility with live tissues, bioengineered tissue patches were created and perfused. After 24 h of perfusion, no significant difference in cell viability was observed between the perfused samples and static controls, indicating no adverse effects on cell health. Perfusion also facilitated enhanced spatial organization within tissue patches, reducing the inter-spheroids distance. Furthermore, perfusion strengthened the tissue matrix and reduced the degradation rate of the hydrogel scaffold. Complemented by its ability to provide mobile perfusion and incubation, this novel integrated portable perfusion system holds promise for promoting tissue maturation and advancing tissue bioengineering studies.
灌注为组织工程系统带来了独特的益处，增强了氧气和营养物质的传输，从而改善了组织的形成和生长。在本研究中，我们展示了一种新型的集成便携式灌注系统。该系统重量小于10磅，能够在标准培养环境（37°C，5%二氧化碳）中维持连续流动，有效地作为便携式灌注和组织培养系统发挥作用。为了表征灌注系统的流动参数，我们测量了一系列压力下的体积流速，发现该系统能够实现1.69至4.6微米/秒的流速，这与体内间质流动相似。计算流体动力学显示，在灌注系统的含样品区域内存在充分发展的层流，有助于确保流体和营养物质的均匀分布。为了研究该系统与活组织的兼容性，制备了生物工程组织贴片并进行灌注。灌注24小时后，灌注样品与静态对照之间的细胞活力未观察到显著差异，表明对细胞健康没有不良影响。灌注还促进了组织贴片内空间组织的增强，减少了球状体之间的距离。此外，灌注强化了组织基质并降低了水凝胶支架的降解速率。这种新型集成便携式灌注系统具有提供移动灌注和培养的能力，有望促进组织成熟并推动组织生物工程研究。
