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Mamm Genome. 2022 Sep;33(3):437-450. doi: 10.1007/s00335-022-09951-2. Epub 2022 Mar 25.
Tissue organoids or "mini organs" can be invaluable tools for understanding health and disease biology, modeling tissue dynamics, or screening potential drug candidates. Effective vascularization of these models is critical for truly representing the in vivo tissue environment. Not only is the formation of a vascular network, and ultimately a microcirculation, essential for proper distribution and exchange of oxygen and nutrients throughout larger organoids, but vascular cells dynamically communicate with other cells to modulate overall tissue behavior. Additionally, interstitial fluid flow, mediated by a perfused microvasculature, can have profound influences on tissue biology. Thus, a truly functionally and biologically relevant organoid requires a vasculature. Here, we review existing strategies for fabricating and incorporating vascular elements and perfusion within tissue organoids.
组织类器官或“微型器官”对于理解健康和疾病生物学、模拟组织动力学或筛选潜在药物候选物可能非常有用。这些模型的有效血管化对于真正代表体内组织环境至关重要。不仅血管网络的形成，最终是微循环的形成，对于较大的类器官中氧气和营养物质的适当分布和交换至关重要，而且血管细胞与其他细胞动态通信以调节整体组织行为。此外，由灌注的微血管介导的间质液流对流经的组织生物学有深远的影响。因此，真正具有功能和生物学相关性的类器官需要血管系统。在这里，我们综述了在组织类器官中构建和纳入血管成分和灌注的现有策略。
