Fabrication of a Spiral Microfluidic Chip for the Mass Production of Lipid Nanoparticles Using Laser Engraving.

A spiral microfluidic chip (SMC) and multi-spiral microfluidic chip (MSMC) for lipid nanoparticle (LNP) production were fabricated using a CO laser engraving method, using perfluoropolyether (PFPE) and poly(ethylene glycol) diacrylate as photopolymerizable base materials. The SMC includes a spiral microchannel that enables rapid fluid mixing, thereby facilitating the production of small and uniform LNPs with a size of 72.82 ± 24.14 nm and a PDI of 0.111 ± 0.011. The MSMC integrates multiple parallel SMC structures, which enables high-throughput LNP production without compromising quality and achieves a maximum production capacity of 960 mL per hour. The LNP fabrication technology using SMC and MSMC has potential applications in the pharmaceutical field due to the ease of chip fabrication, the simplicity and cost-effectiveness of the process, and the ability to produce high-quality LNPs.