Fuxe K, Wikström A C, Okret S, Agnati L F, Härfstrand A, Yu Z Y, Granholm L, Zoli M, Vale W, Gustafsson J A
Endocrinology. 1985 Nov;117(5):1803-12. doi: 10.1210/endo-117-5-1803.
By means of a monoclonal antibody against the rat liver glucocorticoid receptor (GR) in combination with the indirect immunoperoxidase technique it has been possible to demonstrate GR-immunoreactive nerve and glial cell nuclei all over the tel- and diencephalon of the male rat. Strongly GR-immunoreactive nerve cell nuclei were only present in the parvocellular part of the paraventricular hypothalamic nucleus, in the anterior periventricular hypothalamic nucleus, in the ventral part of the mediobasal hypothalamus, and in the CA1 and CA2 subregion of the hippocampal formation. Within the paraventricular hypothalamic nucleus a substantial overlap exists between the GR-immunoreactive area and the CRF-immunoreactive area. Medium to high densities of moderately GR-immunoreactive nerve cell nuclei were present all over the cortical hemispheres. Medium densities of moderately GR-immunoreactive nerve cells were demonstrated in many thalamic nuclei and in the central amygdaloid nucleus. After adrenalectomy the GR immunoreactivity was predominantly located in the pericaryon. Upon acute corticosterone treatment of adrenalectomized male rats, the GR immunoreactivity was again mainly demonstrated in the nerve cell nuclei indicating that corticosterone can translocate GR from the cytoplasm to the cell nuclei. It is suggested that the hypothalamic GR may be involved in the regulation of especially CRF secretion but also in the secretion of other anterior pituitary hormones such as TRH and somatostatin.
通过一种针对大鼠肝脏糖皮质激素受体（GR）的单克隆抗体，结合间接免疫过氧化物酶技术，已能够在雄性大鼠的端脑和间脑各处显示出GR免疫反应性神经细胞核和神经胶质细胞核。强GR免疫反应性神经细胞核仅存在于下丘脑室旁核的小细胞部、室旁下丘脑前核、下丘脑内侧基底部的腹侧部以及海马结构的CA1和CA2亚区。在下丘脑室旁核内，GR免疫反应区和CRF免疫反应区存在大量重叠。中等至高密度的中等GR免疫反应性神经细胞核遍布皮质半球。在许多丘脑核和杏仁中央核中显示出中等密度的中等GR免疫反应性神经细胞。肾上腺切除后，GR免疫反应性主要位于核周体。对肾上腺切除的雄性大鼠进行急性皮质酮处理后，GR免疫反应性再次主要显示在神经细胞核中，表明皮质酮可将GR从细胞质转运至细胞核。有人提出，下丘脑GR可能尤其参与促肾上腺皮质激素释放因子（CRF）分泌的调节，也参与其他垂体前叶激素如促甲状腺激素释放激素（TRH）和生长抑素的分泌调节。
