Yang Chenghuizi, Zhong Weiping, Li Wei, Xia Yunong, Qin Lei, Tang Xianyu, Xia Shitou
Department of Agriculture and Forestry, Hainan Tropical Ocean University, Sanya 572022, China.
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Int J Mol Sci. 2025 May 10;26(10):4569. doi: 10.3390/ijms26104569.
is one of the most extensively cultivated oilseed crops in China, but its yield is significantly impacted by stem rot caused by . Receptor-like proteins (RLPs) and receptor-like kinases (RLKs) play essential roles in plant-pathogen interactions; however, their regulatory mechanisms remain largely unknown in . In this study, we investigated the function of the leucine-rich repeat receptor-like protein RLP-G13-1 in immunity. Previous observations indicated that plants expressing RLP-G13-1 exhibited enhanced resistance to . We hypothesized that RLP-G13-1 mediates pathogen recognition and immune signaling. To test this, we employed mitogen-activated protein kinase (MAPK) activity assays, transgenic overexpression analyses, and pathogen infection assays. Our results demonstrated that RLP-G13-1 recognizes the conserved necrosis- and ethylene-inducing peptide nlp24 derived from , triggering MAPK cascades and subsequent immune responses. Furthermore, protein interaction studies revealed that RLP-G13-1 physically interacts with the receptor-like kinase SOBIR1, which is essential for full antifungal defense activation. These results elucidate the molecular basis of -mediated immunity, providing insights into improving disease resistance in oilseed crops.
是中国种植最广泛的油料作物之一，但其产量受到由 引起的茎腐病的显著影响。类受体蛋白（RLP）和类受体激酶（RLK）在植物与病原体的相互作用中起重要作用；然而，它们在 中的调控机制仍 largely unknown。在本研究中，我们研究了富含亮氨酸重复序列的类受体蛋白RLP-G13-1在 免疫中的功能。先前的观察表明，表达RLP-G13-1的 植物对 表现出增强的抗性。我们假设RLP-G13-1介导病原体识别和免疫信号传导。为了验证这一点，我们采用了丝裂原活化蛋白激酶（MAPK）活性测定、转基因过表达分析和病原体感染测定。我们的结果表明，RLP-G13-1识别源自 的保守坏死和乙烯诱导肽nlp24，触发MAPK级联反应和随后的免疫反应。此外，蛋白质相互作用研究表明，RLP-G13-1与类受体激酶SOBIR1发生物理相互作用，这对于完全激活抗真菌防御至关重要。这些结果阐明了 -介导的免疫的分子基础，为提高油料作物的抗病性提供了见解。
